Using siRNA to uncover novel oncogenic signaling pathways.

Jin Mei Lai*, Chi Ying F. Huang, Chang Han Chen


研究成果: Article同行評審

6 引文 斯高帕斯(Scopus)


Tumor invasion and metastasis are the primary causes of cancer patient mortality, underscoring the need for identification of novel genes and signaling pathways that mediate these prognosis-determining phenomena. To identify and characterize novel lung adenocarcinoma genes associated with lung cancer progression, we created a bioinformatics-based approach that focuses on human cell-cycle-regulated genes that have evolved only in higher organisms but not in lower eukaryotic cells. In siRNA experiments in lung cancer cells, FLJ10540 was identified as one of several novel targets involved in cell migration and invasion. Here, we demonstrate that PI3K inhibition affects FLJ10540-mediated cell migration and invasion and further, that FLJ10540 knockdown ablates AKT-Ser(473) phosphorylation. Taken together, these findings indicate that the FLJ10540/PI3K/AKT pathway may harbor new therapeutic targets for treating invasive lung adenocarcinoma.

頁(從 - 到)231-242
期刊Methods in molecular biology (Clifton, N.J.)
出版狀態Published - 2010


深入研究「Using siRNA to uncover novel oncogenic signaling pathways.」主題。共同形成了獨特的指紋。