For some applications, the CMOS ICs need to be supplied with positive and negative voltage sources for the desired circuit operations. To supply the negative voltage source for circuit operations in the silicon chip with the common p-type substrate grounded, the isolation rings configured with n-well (NW) and deep n-well (DNW) layers must be used to isolate the circuits of nMOS devices operating with negative voltage from the common P-substrate. Such NW/DNW isolation rings in the circuit layouts are often connected to ground (GND =0V) for the circuit operations with negative voltage source. But, a parasitic p-n-p-n path from I/O pMOS to this grounded NW/DNW isolation ring may cause the circuits at high risk to latch-up. In this letter, a novel method to improve latch-up immunity against such parasitic p-n-p-n path by using a Schottky junction is reported.