Using cationic polyurethane-short branch PEI as microRNA-driven nano-delivery system for stem cell differentiation

Chian-Shiu Chien, Chien-Ying Wang, Yi-Ping Yang, Shih-Jie Chou, Yu-Ling Ko, Fu-Ting Tsai, Wen-Chung Yu, Chia-Ching Chang, Jong-Yuh Cherng, Meng-Yin Yang*

*此作品的通信作者

研究成果: Article同行評審

5 引文 斯高帕斯(Scopus)

摘要

Background:

Non-viral gene delivery, such as using biodegradable polyurethane short-branch polyethylenimine (PU-PEI), has been considered a potentially safer gene delivery system in comparison to conventional virus systems.

Methods:

The polycationization of DNA complexes protects DNA from nuclease degradation, and these DNA complexes are nanoscale in size to enter the cell through endocytosis.

Results:

Due to the net positive surface charge of the cell, these polyplexes efficiently bind to the cell through electrostatic interactions with negatively charged membrane components. Cationic PU-PEI has been shown to be non-cytotoxic and has a high transfection efficiency, making it a practical gene delivery material in diseases.

Conclusion:

We developed a PU-PEI nanomedicine-based platform to efficiently deliver microRNA in promoting differentiation capacity of stem cells, especially on induced pluripotent stem cells.

原文English
頁(從 - 到)367-370
頁數4
期刊Journal of the Chinese Medical Association
83
發行號4
DOIs
出版狀態Published - 4月 2020

指紋

深入研究「Using cationic polyurethane-short branch PEI as microRNA-driven nano-delivery system for stem cell differentiation」主題。共同形成了獨特的指紋。

引用此