Upregulation of SOX9 in lung adenocarcinoma and its involvement in the regulation of cell growth and tumorigenicity

Shih Sheng Jiang, Wen Tsen Fang, Ya Hsiue Hou, Shiu Feng Huang, B. Linju Yen, Junn Liang Chang, Shih Miao Li, Hui Ping Liu, Ying Lan Liu, Chih Ting Huang, Yu Wei Li, Te Hsuan Jang, Shih Hsuan Chan, Su Jing Yang, Chao A. Hsiung, Cheng-Wen Wu, Lu Hai Wang, I. Shou Chang*

*此作品的通信作者

研究成果: Article同行評審

100 引文 斯高帕斯(Scopus)

摘要

Purpose: SOX9 is an important transcription factor required for development and has been implicated in several types of cancer. However, SOX9 has never been linked to lung cancer to date. Here, we show that SOX9 expression is upregulated in lung adenocarcinoma and show how it is associated with cancer cell growth. Experimental Design: Data mining with five microarray data sets containing 490 clinical samples, quantitative reverse transcription-PCR validation assay in 57 independent samples, and immunohistochemistry assay with tissue microarrays containing 170 lung tissue cores were used to profile SOX9 mRNA and protein expression. Short interference RNA suppression of SOX9 in cell lines was used to scrutinize functional role(s) of SOX9 and associated molecular mechanisms. Results: SOX9 mRNA and protein were consistently overexpressed in the majority of lung adenocarcinoma. Knockdown of SOX9 in lung adenocarcinoma cell lines resulted in marked decrease of adhesive and anchorage-independent growth in concordance with the upregulation of p21 (CDKN1A) and downregulation of CDK4. In agreement with higher SOX9 expression level in lung adenocarcinoma, the p21 mRNA level was significantly lower in tumors than that in normal tissues, whereas the opposite was true for CDK4, supporting the notion that SOX9 negatively and positively regulated p21 and CDK4, respectively. Finally, whereas SOX9-knockdown cells showed significantly attenuated tumorigenicity in mice, SOX9 transfectants consistently showed markedly stronger tumorigenicity. Conclusions: Our data suggest that SOX9 is a new hallmark of lung adenocarcinoma, in which SOX9 might contribute to gain of tumor growth potential, possibly acting through affecting the expression of cell cycle regulators p21 and CDK4.

原文English
頁(從 - 到)4363-4373
頁數11
期刊Clinical Cancer Research
16
發行號17
DOIs
出版狀態Published - 1 九月 2010

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