Uncovering Listeria monocytogenes hypervirulence by harnessing its biodiversity

Mylène M. Maury, Yu Huan Tsai, Caroline Charlier, Marie Touchon, Viviane Chenal-Francisque, Alexandre Leclercq, Alexis Criscuolo, Charlotte Gaultier, Sophie Roussel, Anne Brisabois, Olivier Disson, Eduardo P.C. Rocha, Sylvain Brisse*, Marc Lecuit

*此作品的通信作者

研究成果: Article同行評審

453 引文 斯高帕斯(Scopus)

摘要

Microbial pathogenesis studies are typically performed with reference strains, thereby overlooking within-species heterogeneity in microbial virulence. Here we integrated human epidemiological and clinical data with bacterial population genomics to harness the biodiversity of the model foodborne pathogen Listeria monocytogenes and decipher the basis of its neural and placental tropisms. Taking advantage of the clonal structure of this bacterial species, we identify clones epidemiologically associated either with food or with human central nervous system (CNS) or maternal-neonatal (MN) listeriosis. The latter clones are also most prevalent in patients without immunosuppressive comorbidities. Strikingly, CNS- and MN-associated clones are hypervirulent in a humanized mouse model of listeriosis. By integrating epidemiological data and comparative genomics, we have uncovered multiple new putative virulence factors and demonstrate experimentally the contribution of the first gene cluster mediating L. monocytogenes neural and placental tropisms. This study illustrates the exceptional power in harnessing microbial biodiversity to identify clinically relevant microbial virulence attributes.

原文English
頁(從 - 到)308-313
頁數6
期刊Nature Genetics
48
發行號3
DOIs
出版狀態Published - 1 3月 2016

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