Uncoiling mechanism of klebsiella pneumoniae type 3 pili measured by using optical tweezers

Feng Jung Chen*, Chia Han Chan, Kuo Liang Liu, Ying Jung Huang, Hwei-Ling Peng, Hwan You Chang, Tri Rung Yew, Ken-Yuh Hsu, Long Hsu

*此作品的通信作者

研究成果: Conference contribution同行評審

1 引文 斯高帕斯(Scopus)

摘要

Pili are bacterial appendages that play many important roles in bacterial behaviors, physiology and interaction with hosts. Via pili, bacteria are able to adhere to, migrate onto, and colonize on host cells, mechanically. Different from the most studied type 1 and P type pili, which are rigid and thick with an average of 6-7 nm in diameter, type 3 pili are relatively tiny (3-5 nm in diameter) and flexible, and their biophysical properties remains unclear. By using optical tweezers, we found that the elongation processes of type 3 pili are divided into three phases: (1) elastic elongation, (2) uncoiling elongation, and (3) intrinsic elongation, separately. Besides, the uncoiling force of the recombinant pili displayed on the surface of E. coli [pmrkABCDv1F] is measured 20 pN in average stronger than that of E. coli [pmrkABCD v1]. This suggests that pilin MrkF is involved in determining the mechanical properties of the type 3 pili.

原文English
主出版物標題Optical Trapping and Optical Micromanipulation IV
DOIs
出版狀態Published - 1 十二月 2007
事件Optical Trapping and Optical Micromanipulation IV - San Diego, CA, United States
持續時間: 26 八月 200729 八月 2007

出版系列

名字Proceedings of SPIE - The International Society for Optical Engineering
6644
ISSN(列印)0277-786X

Conference

ConferenceOptical Trapping and Optical Micromanipulation IV
國家/地區United States
城市San Diego, CA
期間26/08/0729/08/07

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