Two-photon imaging of the immune system

Ivan L. Dzhagalov*, Heather J. Melichar, Jenny O. Ross, Paul Herzmark, Ellen A. Robey

*此作品的通信作者

研究成果: Article同行評審

34 引文 斯高帕斯(Scopus)

摘要

Two-photon microscopy is a powerfulmethod for visualizing biological processes as they occur in their native environment in real time. The immune system uniquely benefits from this technology as most of its constituent cells are highly motile and interact extensively with each other and with the environment. Two-photon microscopy has provided many novel insights into the dynamics of the development and function of the immune system that could not have been deduced by other methods and has become an indispensible tool in the arsenal of immunologists. In this unit, we provide several protocols for preparation of various organs for imaging by two-photon microscopy that are intended to introduce the new user to some basic aspects of this method.

原文English
文章編號12.26
期刊Current Protocols in Cytometry
發行號SUPPL.60
DOIs
出版狀態Published - 4月 2012

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