Transposon mutagenesis identifies genes critical for growth of Pseudomonas nitroreducens TX1 on octylphenol polyethoxylates

Tuan Ngoc Nguyen, Chen Wei Yeh, Po Chun Tsai, Kyoung Lee, Shir Ly Huang*

*此作品的通信作者

研究成果: Article同行評審

12 引文 斯高帕斯(Scopus)

摘要

Pseudomonas nitroreducens TX1 is of special interest because of its ability to utilize 0.05% to 20% octylphenol polyethoxylates (OPEOn) as a sole source of carbon. In this study, a library containing 30,000 Tn5-insertion mutants of the wild-type strain TX1 was constructed and screened for OPEOn utilization, and 93 mutants were found to be unable to grow on OPEOn. In total, 42 separate disrupted genes were identified, and the proteins encoded by the genes were then classified into various categories, namely, information storage and processing (14.3%), cellular processes and signaling (28.6%), metabolism (35.7%), and unknown proteins (21.4%). The individual deletion of genes encoding isocitrate lyase (aceA), malate synthase (aceB), and glycolate dehydrogenase (glcE) was carried out, and the requirement for aceA and aceB but not glcE confirmed the role of the glyoxylate cycle in OPEOn degradation. Furthermore, acetaldehyde dehydrogenase and acetyl-coenzyme A (acetyl-CoA) synthetase activity levels were 13.2- and 2.1-fold higher in TX1 cells grown on OPEOn than in TX1 cells grown on succinate, respectively. Growth of the various mutants on different carbon sources was tested, and based on these findings, a mechanism involving exoscission to liberate acetaldehyde from the end of the OPEOn chain during degradation is proposed for the breakdown of OPEOn.

原文English
頁(從 - 到)6584-6592
頁數9
期刊Applied and Environmental Microbiology
82
發行號22
DOIs
出版狀態Published - 2016

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