Thermal stability and folding kinetics analysis of disordered protein, securin

Hsueh Liang Chu, Tzu Hsuan Chen, Chang You Wu, Yao Chen Yang, Shin Hua Tseng, Tsai Mu Cheng, Li Ping Ho, Li Yun Tsai, Hsing Yuan Li, Chia Seng Chang, Chia-Ching Chang*


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4 引文 斯高帕斯(Scopus)


Lacking a stable tertiary structure, intrinsically disordered proteins (IDPs) possess particular functions in cell regulation, signaling, and controlling pathways. The study of their unique structural features, thermal stabilities, and folding kinetics is intriguing. In this study, an identified IDP, securin, was used as a model protein. By using a quasi-static five-step (on-path) folding process, the function of securin was restored and analyzed by isothermal titration calorimetry. Fluorescence spectroscopy and particle size analysis indicated that securin possessed a compact hydrophobic core and particle size. The glass transition of securin was characterized using differential scanning microcalorimetry. Furthermore, the folding/unfolding rates (kobs) of securin were undetectable, implying that the folding/unfolding rate is very fast and that the conformation of securin is sensitive to solvent environmental change. Therefore, securin may fold properly under specific physiological conditions. In summary, the thermal glass transition behavior and undetectable kobs of folding/unfolding reactions may be two of the indices of IDP.

頁(從 - 到)2171-2178
期刊Journal of Thermal Analysis and Calorimetry
出版狀態Published - 1 3月 2014


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