TY - JOUR
T1 - The Use of Optical Parametric Oscillator for Harmonic Generation and Two-Photon UV Fluorescence Microscopy
AU - Kao, Fu Jen
PY - 2004/2/15
Y1 - 2004/2/15
N2 - Ultrafast lasers have found increasing use in scanning optical microscopy due to their very high peak power in generating multiphoton excitations. A mode-locked Ti:sapphire laser is often employed for such purposes. Together with a synchronously pumped optical parametric oscillator (OPO), the spectral range available can be extended to 1,050-1,300 nm. This broader range available greatly facilitates the excitation of second harmonic generation (SHG) and third harmonic generation (THG) due to better satisfaction of phase matching condition that is achieved with a longer excitation wavelength. Dental sections are then investigated with the contrasts from harmonic generation. In addition, through intra-cavity doubling wavelengths from 525-650 nm are made available for effective two-photon (2-p) excitation with the equivalent photon energy in the UVB range (290-320 nm) and beyond. This new capacity allows UV (auto-) fluorescence excitation and imaging, for example, from some amino acids, such as tyrosine, tryptophan, and glycine.
AB - Ultrafast lasers have found increasing use in scanning optical microscopy due to their very high peak power in generating multiphoton excitations. A mode-locked Ti:sapphire laser is often employed for such purposes. Together with a synchronously pumped optical parametric oscillator (OPO), the spectral range available can be extended to 1,050-1,300 nm. This broader range available greatly facilitates the excitation of second harmonic generation (SHG) and third harmonic generation (THG) due to better satisfaction of phase matching condition that is achieved with a longer excitation wavelength. Dental sections are then investigated with the contrasts from harmonic generation. In addition, through intra-cavity doubling wavelengths from 525-650 nm are made available for effective two-photon (2-p) excitation with the equivalent photon energy in the UVB range (290-320 nm) and beyond. This new capacity allows UV (auto-) fluorescence excitation and imaging, for example, from some amino acids, such as tyrosine, tryptophan, and glycine.
KW - Intra-cavity doubling
KW - Laser scanning microscopy
KW - Optical parametric oscillator
KW - Second harmonic generation
KW - Third harmonic generation
KW - Two-photon excitation
KW - Ultraviolet
UR - http://www.scopus.com/inward/record.url?scp=1642538967&partnerID=8YFLogxK
U2 - 10.1002/jemt.20026
DO - 10.1002/jemt.20026
M3 - Article
C2 - 14755605
AN - SCOPUS:1642538967
SN - 1059-910X
VL - 63
SP - 175
EP - 181
JO - Microscopy Research and Technique
JF - Microscopy Research and Technique
IS - 3
ER -