The therapeutic potential of human umbilical mesenchymal stem cells from wharton’s jelly in the treatment of rat peritoneal dialysis-induced fibrosis

Yu Pei Fan, Ching Chih Hsia, Kuang Wen Tseng, Chih Kai Liao, Tz Win Fu, Tsui Ling Ko, Mei Miao Chiu, Yang Hsin Shih, Pei Yu Huang, Yi Chia Chiang, Chih Ching Yang, Yu Show Fu*

*此作品的通信作者

研究成果: Article同行評審

22 引文 斯高帕斯(Scopus)

摘要

A major complication in continuous, ambulatory peritoneal dialysis in patients with endstage renal disease who are undergoing long-term peritoneal dialysis (PD) is peritoneal fibrosis, which can result in peritoneal structural changes and functional ultrafiltration failure. Human umbilical mesenchymal stem cells (HUMSCs) in Wharton’s jelly possess stem cell properties and are easily obtained and processed. This study focuses on the effects of HUMSCs on peritoneal fibrosis in in vitro and in vivo experiments. After 24-hour treatment with mixture of Dulbecco’s modified Eagle’s medium and PD solution at a 1:3 ratio, primary human peritoneal mesothelial cells became susceptible to PD-induced cell death. Such cytotoxic effects were prevented by coculturing with primary HUMSCs. In a rat model, intraperitoneal injections of 20 mM methylglyoxal (MGO) in PD solution for 3 weeks (the PD/MGO 3W group) markedly induced abdominal cocoon formation, peritoneal thickening, and collagen accumulation. Immunohistochemical analyses indicated neoangiogenesis and significant increase in the numbers of ED-1- and a-smooth muscle actin (a-SMA)-positive cells in the thickened peritoneum in the PD/MGO 3W group, suggesting that PD/MGO induced an inflammatory response. Furthermore, PD/MGO treatment for 3 weeks caused functional impairments in the peritoneal membrane. However, in comparison with the PD/MGO group, intraperitoneal administration of HUMSCs into the rats significantly ame- liorated the PD/MGO-induced abdominal cocoon formation, peritoneal fibrosis, inflamma- tion, neoangiogenesis, and ultrafiltration failure. After 3 weeks of transplantation, surviving HUMSCs were found in the peritoneum in the HUMSC-grafted rats. Thus, xenografts of HUMSCs might provide a potential therapeutic strategy in the prevention of peritoneal fibrosis.

原文English
頁(從 - 到)235-247
頁數13
期刊Stem cells translational medicine
5
發行號2
DOIs
出版狀態Published - 2016

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