The 1.5 Å structure of endo-1,3-β-glucanase from Streptomyces sioyaensis: Evolution of the active-site structure for 1,3-β-glucan-binding specificity and hydrolysis

Tang Yao Hong, Yu-Yuan Hsiao, Menghsiao Meng, Tien Hsiung Thomas Li

研究成果: Article同行評審

14 引文 斯高帕斯(Scopus)

摘要

The catalytic domain structure of Streptomyces sioyaensis 1,3-β-glucanase (278 amino acids), a member of glycosyl hydrolase family 16 (GHF16), was determined to 1.5 Å resolution in space group P212121. The enzyme specifically hydrolyzes the glycosidic bond of the 1,3-β-linked glucan substrate. The overall structure contains two antiparallel six-and seven-stranded β-sheets stacked in a β-sandwich jelly-roll motif similar to the fold of GHF16 1,3-1,4-β-glucanases. The active-site cleft of the enzyme is distinct, with the closure of one end primarily caused by two protruding loop insertions and two key residues, Tyr38 and Tyr134. The current known structures of 1,3-1,4-β-glucanases and 1,3-β-glucanase from Nocardiopsis sp., on the other hand, have open-channel active-site clefts that can accommodate six β-d-glucopyranosyl units. The active-site structure of 1,3-β-glucanase was compared with those of other homologous structures in order to address the binding and enzymatic specificity for 1,3-β-linked glucans in Streptomyces. This information could be helpful in the development of specific antifungal agents.

原文English
頁(從 - 到)964-970
頁數7
期刊Acta Crystallographica Section D: Biological Crystallography
64
發行號9
DOIs
出版狀態Published - 13 八月 2008

指紋

深入研究「The 1.5 Å structure of endo-1,3-β-glucanase from Streptomyces sioyaensis: Evolution of the active-site structure for 1,3-β-glucan-binding specificity and hydrolysis」主題。共同形成了獨特的指紋。

引用此