Telomeric proteins regulate episomal maintenance of epstein-barr virus origin of plasmid replication

Zhong Deng, Larissa Lezina, Chi Ju Chen, Svetlana Shtivelband, Wingkan So, Paul M. Lieberman

研究成果: Article同行評審

118 引文 斯高帕斯(Scopus)

摘要

Episomal maintenance and DNA replication of EBV origin of plasmid replication (OriP) plasmid maintenance is mediated by the viral encoded origin binding protein, EBNA1, and unknown cellular factors. We found that telomeric repeat binding factor 2 (TRF2), TRF2-interacting protein hRap1, and the telomere-associated poly(ADP-ribose) polymerase (Tankyrase) bound to the dyad symmetry (DS) element of OriP in an EBNA1-dependent manner. TRF2 bound cooperatively with EBNA1 to the three nonamer sites (TTAGGGTTA), which resemble telomeric repeats. Mutagenesis of the nonamers reduced plasmid maintenance function and increased plasmid sensitivity to genotoxic stress. DS affinity-purified proteins possessed poly(ADP-ribose) polymerase (PARP) activity, and EBNA1 was subject to NAD-dependent posttranslational modification in vitro. OriP plasmid maintenance was sensitive to changes in cellular PARP/Tankyrase activity. These findings imply that telomere-associated proteins regulate OriP plasmid maintenance by PAR-dependent modifications.

原文English
頁(從 - 到)493-503
頁數11
期刊Molecular Cell
9
發行號3
DOIs
出版狀態Published - 2002

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