TY - JOUR
T1 - Surface modification of nanoporous poly(ε-caprolactone) membrane with poly(ethylene glycol) to prevent biofouling
T2 - Part I. Effects of plasma power and treatment time
AU - Yen, Chi
AU - He, Hongyan
AU - Fei, Zhengzheng
AU - Zhang, Xulang
AU - Lee, L. James
AU - Ho, W. S.Winston
N1 - Funding Information:
1William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio, United States 2NSF Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, Ohio, United States 3Department of Materials Science and Engineering, The Ohio State University, Columbus, Ohio, United States Biofouling, a result of protein adsorption and cell adhesion on a surface, is detrimental to membrane performance. The objective of this study is to modify the polycaprolactone (PCL) membrane surface with poly(ethylene glycol) (PEG) to prevent fibroblast adhesion. To achieve this goal, oxygen plasma and PEG(400)-monoacrylate were used Received 11 March 2010; accepted 26 May 2010. We would like to thank the National Science Foundation-sponsored Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices (NSEC-CANPBD) at the Ohio State University for the financial support of this work. Address correspondence to W. S. Winston Ho, William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio, United States. E-mail: [email protected] to graft the PEG onto the membrane surface through covalent bonding. Various plasma treatment conditions were investigated to optimize the PEG-grafting quality and to achieve minimum fibroblast adhesion. After the treatment, the water contact angle decreased significantly. Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra indicated that PEG was successfully grafted onto the PCL membrane with the appearance of the PEG characteristic peaks. X-ray photoelectron spectroscopy (XPS) revealed that different plasma powers and treatment times changed the surface composition of membranes. To evaluate the applicability of this new strategy for the prevention of biofouling, NIH 3T3 fibroblast was used as a model biofoulant. Cell adhesion and morphology studies indicate that either lower plasma power or shorter treatment time is able to improve resistance to the cell adhesion. This simple and efficient method can be applied to inhibit biofouling on the membrane surface.
PY - 2010/11
Y1 - 2010/11
N2 - Biofouling, a result of protein adsorption and cell adhesion on a surface, is detrimental to membrane performance. The objective of this study is to modify the polycaprolactone (PCL) membrane surface with poly(ethylene glycol) (PEG) to prevent fibroblast adhesion. To achieve this goal, oxygen plasma and PEG(400)-monoacrylate were used to graft the PEG onto the membrane surface through covalent bonding. Various plasma treatment conditions were investigated to optimize the PEG-grafting quality and to achieve minimum fibroblast adhesion. After the treatment, the water contact angle decreased significantly. Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra indicated that PEG was successfully grafted onto the PCL membrane with the appearance of the PEG characteristic peaks. X-ray photoelectron spectroscopy (XPS) revealed that different plasma powers and treatment times changed the surface composition of membranes. To evaluate the applicability of this new strategy for the prevention of biofouling, NIH 3T3 fibroblast was used as a model biofoulant. Cell adhesion and morphology studies indicate that either lower plasma power or shorter treatment time is able to improve resistance to the cell adhesion. This simple and efficient method can be applied to inhibit biofouling on the membrane surface.
AB - Biofouling, a result of protein adsorption and cell adhesion on a surface, is detrimental to membrane performance. The objective of this study is to modify the polycaprolactone (PCL) membrane surface with poly(ethylene glycol) (PEG) to prevent fibroblast adhesion. To achieve this goal, oxygen plasma and PEG(400)-monoacrylate were used to graft the PEG onto the membrane surface through covalent bonding. Various plasma treatment conditions were investigated to optimize the PEG-grafting quality and to achieve minimum fibroblast adhesion. After the treatment, the water contact angle decreased significantly. Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra indicated that PEG was successfully grafted onto the PCL membrane with the appearance of the PEG characteristic peaks. X-ray photoelectron spectroscopy (XPS) revealed that different plasma powers and treatment times changed the surface composition of membranes. To evaluate the applicability of this new strategy for the prevention of biofouling, NIH 3T3 fibroblast was used as a model biofoulant. Cell adhesion and morphology studies indicate that either lower plasma power or shorter treatment time is able to improve resistance to the cell adhesion. This simple and efficient method can be applied to inhibit biofouling on the membrane surface.
KW - biofouling
KW - oxygen plasma
KW - poly(ethylene glycol) (PEG)
KW - polycaprolactone (PCL) membrane
KW - surface modification
UR - http://www.scopus.com/inward/record.url?scp=77957252280&partnerID=8YFLogxK
U2 - 10.1080/00914037.2010.504162
DO - 10.1080/00914037.2010.504162
M3 - Article
AN - SCOPUS:77957252280
SN - 0091-4037
VL - 59
SP - 923
EP - 942
JO - International Journal of Polymeric Materials and Polymeric Biomaterials
JF - International Journal of Polymeric Materials and Polymeric Biomaterials
IS - 11
ER -