Surface enhanced FTIR spectroscopy and surface plasmon resonance on biomembranes

Gerald Steiner; Reiner Salzer; Wolfgang Fischer; Christian Kuhne

doi:10.1117/12.384946

Surface enhanced FTIR spectroscopy and surface plasmon resonance on biomembranes

Gerald Steiner^*, Reiner Salzer, Wolfgang Fischer, Christian Kuhne

^*此作品的通信作者

生醫光電研究所

研究成果: Conference article › 同行評審

2 引文斯高帕斯（Scopus）

摘要

Lipid bilayers containing the nicotinic acetylcholine receptor were investigated by attenuated total reflection (ATR) FTIR spectroscopy and by surface plasmon resonance (SPR). FTIR-ATR spectra reveal structural details of the transmembrane protein. Surface enhanced infrared absorption (SEIRA) provides access to structural details in the close vicinity of the surface of the ATR crystal. Information about the abundance of protein substructures as well as about the orientation of those substructures are obtained. SPR was applied in order to detect changes within the adsorbed biomembrane during admission of a neurotransmitter as acetylcholine. Switching of the ion channel was successfully observed by SPR. This result is a step forward towards highly selective biosensors and bioactuators.

原文	English
頁（從 - 到）	215-221
頁數	7
期刊	Proceedings of SPIE - The International Society for Optical Engineering
卷	3918
DOIs	https://doi.org/10.1117/12.384946
出版狀態	Published - 2000
事件	Biomedical Spectroscopy: Vibrational Spectroscopy and other Novel Techniques - San Jose, CA, USA 持續時間: 26 1月 2000 → 27 1月 2000

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title = "Surface enhanced FTIR spectroscopy and surface plasmon resonance on biomembranes",

abstract = "Lipid bilayers containing the nicotinic acetylcholine receptor were investigated by attenuated total reflection (ATR) FTIR spectroscopy and by surface plasmon resonance (SPR). FTIR-ATR spectra reveal structural details of the transmembrane protein. Surface enhanced infrared absorption (SEIRA) provides access to structural details in the close vicinity of the surface of the ATR crystal. Information about the abundance of protein substructures as well as about the orientation of those substructures are obtained. SPR was applied in order to detect changes within the adsorbed biomembrane during admission of a neurotransmitter as acetylcholine. Switching of the ion channel was successfully observed by SPR. This result is a step forward towards highly selective biosensors and bioactuators.",

author = "Gerald Steiner and Reiner Salzer and Wolfgang Fischer and Christian Kuhne",

year = "2000",

doi = "10.1117/12.384946",

language = "English",

volume = "3918",

pages = "215--221",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

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note = "Biomedical Spectroscopy: Vibrational Spectroscopy and other Novel Techniques ; Conference date: 26-01-2000 Through 27-01-2000",

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TY - JOUR

T1 - Surface enhanced FTIR spectroscopy and surface plasmon resonance on biomembranes

AU - Steiner, Gerald

AU - Salzer, Reiner

AU - Fischer, Wolfgang

AU - Kuhne, Christian

PY - 2000

Y1 - 2000

N2 - Lipid bilayers containing the nicotinic acetylcholine receptor were investigated by attenuated total reflection (ATR) FTIR spectroscopy and by surface plasmon resonance (SPR). FTIR-ATR spectra reveal structural details of the transmembrane protein. Surface enhanced infrared absorption (SEIRA) provides access to structural details in the close vicinity of the surface of the ATR crystal. Information about the abundance of protein substructures as well as about the orientation of those substructures are obtained. SPR was applied in order to detect changes within the adsorbed biomembrane during admission of a neurotransmitter as acetylcholine. Switching of the ion channel was successfully observed by SPR. This result is a step forward towards highly selective biosensors and bioactuators.

AB - Lipid bilayers containing the nicotinic acetylcholine receptor were investigated by attenuated total reflection (ATR) FTIR spectroscopy and by surface plasmon resonance (SPR). FTIR-ATR spectra reveal structural details of the transmembrane protein. Surface enhanced infrared absorption (SEIRA) provides access to structural details in the close vicinity of the surface of the ATR crystal. Information about the abundance of protein substructures as well as about the orientation of those substructures are obtained. SPR was applied in order to detect changes within the adsorbed biomembrane during admission of a neurotransmitter as acetylcholine. Switching of the ion channel was successfully observed by SPR. This result is a step forward towards highly selective biosensors and bioactuators.

UR - http://www.scopus.com/inward/record.url?scp=0033701167&partnerID=8YFLogxK

U2 - 10.1117/12.384946

DO - 10.1117/12.384946

M3 - Conference article

AN - SCOPUS:0033701167

SN - 0277-786X

VL - 3918

SP - 215

EP - 221

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Biomedical Spectroscopy: Vibrational Spectroscopy and other Novel Techniques

Y2 - 26 January 2000 through 27 January 2000

ER -

Surface enhanced FTIR spectroscopy and surface plasmon resonance on biomembranes

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