Suppressive effect of carotenoid extract of Dunaliella salina alga on production of LPS-stimulated pro-inflammatory mediators in RAW264.7 cells via NF-κB and JNK inactivation

Deng Jye Yang, Jau Tien Lin, Yi Chen Chen, Shih Chuan Liu, Fung Jou Lu, Tien Jye Chang, Meilin Wang, Hui Wen Lin*, Yuan Yen Chang

*此作品的通信作者

研究成果: Article同行評審

58 引文 斯高帕斯(Scopus)

摘要

The carotenoid extract from Dunaliella salina was used to evaluate the suppressive effects on lipopolysaccharide (LPS)-induced pro-inflammatory mediators in RAW264.7 cells. The extract composed all-trans forms of α-carotene (28.8mg/g extract), β-carotene (471.1mg/g extract), lutein (7.1mg/g extract) and zeaxanthin (7.2mg/g extract), 13- or 13'-cis-β-carotene (12.1mg/g extract), 9- or 9'-cis-α-carotene (19.1mg/g extract) and 9- or 9'-cis-β-carotene (440.3mg/g extract) dose-dependently reduced the production of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α), the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and the secretion of nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-activated RAW264.7 cells. Its attenuation of LPS-induced inflammatory responses was closely related to inhibition of the nuclear NF-κB p50 subunit translocation by blocking inhibitor of κBα (IκB) phosphorylation and degradation correlated with suppressing IκB kinase (IKK) α/β phosphorylation, as well as down-regulation of the c-Jun NH2-terminal kinase (JNK) activation.

原文English
頁(從 - 到)607-615
頁數9
期刊Journal of Functional Foods
5
發行號2
DOIs
出版狀態Published - 4月 2013

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