Study of the reactive excited-state dynamics of delipidated bacteriorhodopsin upon surfactant treatments

Chi Wen Cheng, Yuan-Pern Lee*, Li Kang Chu

*此作品的通信作者

研究成果: Article同行評審

6 引文 斯高帕斯(Scopus)

摘要

The dynamics of the reactive excited-state of bacteriorhodopsin treated with various surfactants have been investigated through the measurement of pump-probe absorption spectra. Upon excitation of bR at 570 nm in the presence of ionic surfactants CTAB and SDS, the lifetime of the reactive excited-state of the all-trans protonated Schiff base, probed at 460 nm, was observed to increase up to 20%, whereas an insignificant change was observed upon addition of neutral surfactant C6E2. Measurements of steady-state absorption spectra, fluorescence, and circular dichroism indicated that the bR suspensions retain their trimeric configuration with partial delipidation. This removal of lipids causes a structural alteration and the varied excited-state dynamics.

原文English
頁(從 - 到)151-156
頁數6
期刊Chemical Physics Letters
539-540
DOIs
出版狀態Published - 29 6月 2012

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