Stretching submicron biomolecules with constant-force axial optical tweezers

Yih Fan Chen, Gerhard A. Blab, Jens Christian Meiners*

*此作品的通信作者

研究成果: Article同行評審

47 引文 斯高帕斯(Scopus)

摘要

Optical tweezers have become powerful tools to manipulate biomolecular systems, but are increasingly difficult to use when the size of the molecules is <1 μm. Many important biological structures and processes, however, occur on the submicron length scale. Therefore, we developed and characterized an optical manipulation protocol that makes this length scale accessible by stretching the molecule in the axial direction of the laser beam, thus avoiding limiting artifacts from steric hindrances from the microscope coverslip and other surface effects. The molecule is held under constant mechanical tension by a combination of optical gradient forces and backscattering forces, eliminating the need for electronic feedback. We demonstrate the utility of this method through a measurement of the force-extension relationship of a 1298 bp ds-DNA molecule.

原文English
頁(從 - 到)4701-4708
頁數8
期刊Biophysical Journal
96
發行號11
DOIs
出版狀態Published - 2009

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