Spherical aberration cancellation in a polarized photon-pairs confocal laser scanning microscope

Chi Hui Chang*, Chien Chou, Hsiu Fong Chang, Hon Fai Yau, Huan Jang Huang, Wen Chuan Kuo

*此作品的通信作者

研究成果: Conference contribution同行評審

摘要

Refractive-index mismatch in conventional confocal microscopy produces severe degradation on axial resolution of sectioning image because the spherical aberration is generated in specimen. In this study, we propose a polarized photon-pairs confocal laser scanning microscope (PCLSM) in which a two-frequency linear polarized photon-pairs (LPPPs) laser beam is produced. The common-path propagation of LPPPs integrated with optical heterodyne technique not only can reduce the spherical aberration but also decreases scattering effect in specimen at same time. Therefore, the better axial and lateral resolutions of the sectioning image are produced simultaneously. In the experiment, a verification and comparison between PCLSM and conventional confocal laser scanning microscope (CLSM) on the ability of cancellation of spherical aberration induced by cover glass are demonstrated experimentally. Finally, the ability of PCLSM which can decrease the spherical aberration based on the common-path propagation of LPPPs associated with polarization gating, spatial coherence gating and spatial filtering gating is discussed.

原文English
主出版物標題Confocal, Multiphoton, and Nonlinear Microscopic Imaging III
發行者Optical Society of America
ISBN(列印)081946774X, 9780819467744
DOIs
出版狀態Published - 6月 2007
事件European Conference on Biomedical Optics, ECBO 2007 - Munich, Germany
持續時間: 17 6月 200717 6月 2007

出版系列

名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE
6630
ISSN(列印)1605-7422

Conference

ConferenceEuropean Conference on Biomedical Optics, ECBO 2007
國家/地區Germany
城市Munich
期間17/06/0717/06/07

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