Spatiotemporal characterization of phagocytic NADPH oxidase and oxidative destruction of intraphagosomal organisms in vivo using autofluorescence imaging and raman microspectroscopy

Wei Tien Chang, Yi Cyun Yang, Hsueh H. Lu, I. L. Li, Ian Liau*

*此作品的通信作者

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12 引文 斯高帕斯(Scopus)

摘要

(Figure Presented) The ability to generate antimicrobial reactive-oxygen species (ROS) by reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is critical for the host to defend against invading microbes. We have demonstrated the application of confocal autofluorescence microscopy and Raman microspectroscopy to characterize dynamically the phagocytic process of living macrophages in a label-free manner. In particular, we visualized the translocation of NADPH oxidases in living macrophages that are undergoing phagocytosis of invading yeasts and monitored dynamically the change at the molecular level of single intraphagosomal yeasts caused by phagocytic ROS.

原文English
頁(從 - 到)1744-1745
頁數2
期刊Journal of the American Chemical Society
132
發行號6
DOIs
出版狀態Published - 1 3月 2010

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