SirT1-A sensor for monitoring self-renewal and aging process in retinal stem cells

Chi Hsien Peng, Yuh Lih Chang, Chung Lan Kao, Ling Ming Tseng, Chih Chia Wu, Yu Chih Chen, Ching Yao Tsai, Lin Chung Woung, Jorn Hon Liu, Shih Hwa Chiou*, Shih Jen Chen

*此作品的通信作者

研究成果: Article同行評審

40 引文 斯高帕斯(Scopus)

摘要

Retinal stem cells bear potency of proliferation, self-renewal, and differentiation into many retinal cells. Utilizing appropriate sensors one can effectively detect the self-renewal and aging process abilities. Silencing information regulator (SirT1), a member of the sirtuin family, is a NAD-dependent histone deacetylase and an essential mediator for longevity in normal cells by calorie restriction. We firstly investigate the SirT1 mRNA expression in retinal stem cells from rats and 19 human eyes of different ages. Results revealed that SirT1 expression was significantly decreased in in vivo aged eyes, associated with poor self-renewal abilities. Additionally, SirT1 mRNA levels were dose-dependently increased in resveratrol- treated retinal stem cells. The expression of SirT1 on oxidative stress-induced damage was significantly decreased, negatively correlated with the level of intracellular reactive oxygen species production. Treatment with resveratrol could effectively further reduce oxidative stress induced by H2O2 treatment in retinal stem cells. Importantly, the anti-oxidant effects of resveratrol in H2O2-treated retinal stem cells were significantly abolished by knockdown of SirT1 expression (sh-SirT1). SirT1 expression provides a feasible sensor in assessing self-renewal and aging process in retinal stem cells. Resveratrol can prevent reactive oxygen species-induced damages via increased retinal SirT1 expression.

原文English
頁(從 - 到)6172-6194
頁數23
期刊Sensors
10
發行號6
DOIs
出版狀態Published - 6月 2010

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