Silencing estrogen receptor-β with siRNA in cultured cells

Ren Jun Hsu, Jui Ming Liu

研究成果: Chapter同行評審

摘要

Estrogen receptors α and β (ERα and ERβ) are the two genomic estrogen receptors. ERβ was the second of these receptors to be discovered; its structure is similar to that of ERα but they are different in histological distribution. However, the functions of ERα versus ERβ are still unclear. The ability of small interfering RNAs (siRNAs) to silence gene expression has proven to be invaluable for studying gene function in cultured mammalian cells. This chapter describes the use of siRNA to inhibit the expression of ERβ in renal cell carcinoma (RCC) and to further the understanding of ERβ function in RCC.

原文English
主出版物標題Methods in Molecular Biology
發行者Humana Press Inc.
頁面353-358
頁數6
DOIs
出版狀態Published - 1 1月 2016

出版系列

名字Methods in Molecular Biology
1366
ISSN(列印)1064-3745

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