Sequence alignment and homology search with BLAST and ClustalW

Jui-Hung Hung, Zhiping Weng

研究成果: Article同行評審

36 引文 斯高帕斯(Scopus)

摘要

This protocol illustrates the steps of searching a sequence database with BLAST, which in essence performs pairwise alignment between the query sequence and each target sequence in the database. Steps for performing multiple sequence alignments with ClustalW are then described. Argonaute 2 (Ago2) is an essential component for small interfering RNA (siRNA)-directed RNA interference (RNAi) in fruit flies. This protocol uses Ago2 to demonstrate how to search for all Ago2 paralogs in the fly proteome using BLAST and to obtain a multiple sequence alignment of these sequences with ClustalW.

原文English
頁(從 - 到)1016-1021
頁數6
期刊Cold Spring Harbor Protocols
2016
發行號11
DOIs
出版狀態Published - 1 十一月 2016

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