Sensitivity enhancement of magneto-optical Faraday effect immunoassay method based on biofunctionalized γ-Fe₂O₃@Au core-shell magneto-plasmonic nanoparticles for the blood detection of Alzheimer's disease

In this work, we conducted a proof-of-concept experiment based on biofunctionalized magneto-plasmonic nanoparticles (MPNs) and magneto-optical Faraday effect for in vitro Alzheimer's disease (AD) assay. The biofunctionalized γ-Fe₂O₃@Au MPNs of which the surfaces are modified with the antibody of Tau protein (anti-τ). As anti-τ reacts with Tau protein, biofunctionalized MPNs aggregate to form magnetic clusters which will hence induce the change of the reagent's Faraday rotation angle. The result showed that the γ-Fe₂O₃@Au core-shell MPNs can enhance the Faraday rotation with respect to the raw γ-Fe₂O₃ nanoparticles. Because of their magneto-optical enhancement effect, biofunctionalized γ-Fe₂O₃@Au MPNs effectively improve the detection sensitivity. The detection limit of Tau protein as low as 9 pg/mL (9 ppt) was achieved. Furthermore, the measurements of the clinical samples from AD patients agreed with the CDR evaluated by the neurologist. The results suggest that our method has the potential for disease assay applications.