Selective and absolute quantification of endogenous hypochlorous acid with quantum-dot conjugated microbeads

Yi Cyun Yang, Hsueh Han Lu, Wei Ti Wang, Ian Liau*

*此作品的通信作者

研究成果: Article同行評審

51 引文 斯高帕斯(Scopus)

摘要

Endogenous hypochlorous acid (HOCl) secreted by leukocytes plays a critical role in both the immune defense of mammalians and the pathogenesis of various diseases intimately related to inflammation. We report the first selective and absolute quantification of endogenous HOCl produced by leukocytes in vitro and in vivo with a novel quantum dot-based sensor. An activated human neutrophil secreted 6.5 ± 0.9 × 10 8 HOCl molecules into its phagosome, and kinetic measurement for the secretions showed that the extracellular generation of HOCl was temporally retarded, but the quantity eventually attained a level comparable with its intraphagosomal counterpart with a delay of about 1.5 h. The quantity of HOCl secreted from the hepatic leukocytes of rats with or without stimulation of lipopolysaccharide was also determined. These results indicate a possibility to extend our approach to not only clinical settings for quantitative assessment of the bactericidal capability of isolated leukocytes of patients but also fundamental biomedical research that requires critical evaluation of the inflammatory response of animals.

原文English
頁(從 - 到)8267-8272
頁數6
期刊Analytical Chemistry
83
發行號21
DOIs
出版狀態Published - 1 11月 2011

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