Selection of an effective red-pigment producing Monascus pilosus by efficient transformation with aurintricarboxylic acid

Yi Pei Chen; I. Ching Chen; Ing Er Hwang; Gwo Fang Yuan; Li Ling Liaw; Ching-Ping Tseng

doi:10.1271/bbb.80342

Selection of an effective red-pigment producing Monascus pilosus by efficient transformation with aurintricarboxylic acid

Yi Pei Chen, I. Ching Chen, Ing Er Hwang, Gwo Fang Yuan, Li Ling Liaw, Ching-Ping Tseng^*

^*此作品的通信作者

生物科技學系

研究成果: Article › 同行評審

5 引文斯高帕斯（Scopus）

摘要

The filamentous fungus Monascus pilosus was genetically transformed with a reporter plasmid, pMS-1.5hp, by aurintricarboxylic acid (ATA) treatment to obtain an efficient red-pigment producing mutant. The transformation efficiency of Monascus pilosus was higher with the ATA-treatment than with either a non-restriction-enzyme-mediated integration (REMI) or a REMI method. This valid and convenient random mutagenesis method shows that ATA can be applied in fungi for efficient genetic transformation.

原文	English
頁（從 - 到）	3021-3024
頁數	4
期刊	Bioscience, Biotechnology and Biochemistry
卷	72
發行號	11
DOIs	https://doi.org/10.1271/bbb.80342
出版狀態	Published - 2008

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abstract = "The filamentous fungus Monascus pilosus was genetically transformed with a reporter plasmid, pMS-1.5hp, by aurintricarboxylic acid (ATA) treatment to obtain an efficient red-pigment producing mutant. The transformation efficiency of Monascus pilosus was higher with the ATA-treatment than with either a non-restriction-enzyme-mediated integration (REMI) or a REMI method. This valid and convenient random mutagenesis method shows that ATA can be applied in fungi for efficient genetic transformation.",

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author = "Chen, {Yi Pei} and Chen, {I. Ching} and Hwang, {Ing Er} and Yuan, {Gwo Fang} and Liaw, {Li Ling} and Ching-Ping Tseng",

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AU - Chen, Yi Pei

AU - Chen, I. Ching

AU - Hwang, Ing Er

AU - Yuan, Gwo Fang

AU - Liaw, Li Ling

AU - Tseng, Ching-Ping

PY - 2008

Y1 - 2008

N2 - The filamentous fungus Monascus pilosus was genetically transformed with a reporter plasmid, pMS-1.5hp, by aurintricarboxylic acid (ATA) treatment to obtain an efficient red-pigment producing mutant. The transformation efficiency of Monascus pilosus was higher with the ATA-treatment than with either a non-restriction-enzyme-mediated integration (REMI) or a REMI method. This valid and convenient random mutagenesis method shows that ATA can be applied in fungi for efficient genetic transformation.

AB - The filamentous fungus Monascus pilosus was genetically transformed with a reporter plasmid, pMS-1.5hp, by aurintricarboxylic acid (ATA) treatment to obtain an efficient red-pigment producing mutant. The transformation efficiency of Monascus pilosus was higher with the ATA-treatment than with either a non-restriction-enzyme-mediated integration (REMI) or a REMI method. This valid and convenient random mutagenesis method shows that ATA can be applied in fungi for efficient genetic transformation.

KW - Aurintricarboxylic acid (ATA)

KW - Monascus pilosus

KW - Restriction-enzyme-mediated integration (REMI)

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SN - 0916-8451

VL - 72

SP - 3021

EP - 3024

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 11

ER -

Selection of an effective red-pigment producing Monascus pilosus by efficient transformation with aurintricarboxylic acid

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