Safety Evaluation and Anti-Inflammatory Efficacy of Lacticaseibacillus paracasei PS23

Chin Hao Li; Tai Ying Chen; Chien Chen Wu; Shih Hsuan Cheng; Min Yu Chang; Wei Hong Cheng; Shih Hau Chiu; Chien Chi Chen; Ying Chieh Tsai; Deng Jye Yang; Jaw Jou Kang; Po Lin Liao

doi:10.3390/ijms24010724

Safety Evaluation and Anti-Inflammatory Efficacy of Lacticaseibacillus paracasei PS23

Chin Hao Li, Tai Ying Chen, Chien Chen Wu, Shih Hsuan Cheng, Min Yu Chang, Wei Hong Cheng, Shih Hau Chiu, Chien Chi Chen, Ying Chieh Tsai, Deng Jye Yang, Jaw Jou Kang, Po Lin Liao^*

^*此作品的通信作者

食品安全及健康風險評估研究所

研究成果: Article › 同行評審

摘要

Lacticaseibacillus paracasei strain PS23 (PS23) exhibits some probiotic properties. In this study, a genomic analysis of PS23 revealed no genes related to virulence or antibiotic resistance. Moreover, ornithine decarboxylase activity was not detected in vitro. In addition, PS23 was sensitive to the tested antibiotics. Genotoxicity tests for PS23 including the Ames test and chromosomal aberrations in vitro using Chinese hamster ovary cells and micronuclei in immature erythrocytes of ICR mice were all negative. Moreover, following a 28-day study involving repeated oral dose toxicity tests (40, 400, and 4000 mg/kg equal 1.28 × 10¹⁰, 1.28 × 10¹¹, and 1.28 × 10¹² CFU/kg body weight, respectively) using an ICR mouse model, no adverse effects were observed from any doses. In addition, supplementation with live or heat-killed PS23 ameliorates DSS-induced colonic inflammation in mice. Our findings suggest that PS23 is safe and has anti-inflammatory effects and may therefore have therapeutic implications.

原文	English
文章編號	724
期刊	International Journal Of Molecular Sciences
卷	24
發行號	1
DOIs	https://doi.org/10.3390/ijms24010724
出版狀態	Published - 1月 2023

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title = "Safety Evaluation and Anti-Inflammatory Efficacy of Lacticaseibacillus paracasei PS23",

abstract = "Lacticaseibacillus paracasei strain PS23 (PS23) exhibits some probiotic properties. In this study, a genomic analysis of PS23 revealed no genes related to virulence or antibiotic resistance. Moreover, ornithine decarboxylase activity was not detected in vitro. In addition, PS23 was sensitive to the tested antibiotics. Genotoxicity tests for PS23 including the Ames test and chromosomal aberrations in vitro using Chinese hamster ovary cells and micronuclei in immature erythrocytes of ICR mice were all negative. Moreover, following a 28-day study involving repeated oral dose toxicity tests (40, 400, and 4000 mg/kg equal 1.28 × 1010, 1.28 × 1011, and 1.28 × 1012 CFU/kg body weight, respectively) using an ICR mouse model, no adverse effects were observed from any doses. In addition, supplementation with live or heat-killed PS23 ameliorates DSS-induced colonic inflammation in mice. Our findings suggest that PS23 is safe and has anti-inflammatory effects and may therefore have therapeutic implications.",

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author = "Li, {Chin Hao} and Chen, {Tai Ying} and Wu, {Chien Chen} and Cheng, {Shih Hsuan} and Chang, {Min Yu} and Cheng, {Wei Hong} and Chiu, {Shih Hau} and Chen, {Chien Chi} and Tsai, {Ying Chieh} and Yang, {Deng Jye} and Kang, {Jaw Jou} and Liao, {Po Lin}",

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AU - Li, Chin Hao

AU - Chen, Tai Ying

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AU - Cheng, Shih Hsuan

AU - Chang, Min Yu

AU - Cheng, Wei Hong

AU - Chiu, Shih Hau

AU - Chen, Chien Chi

AU - Tsai, Ying Chieh

AU - Yang, Deng Jye

AU - Kang, Jaw Jou

AU - Liao, Po Lin

PY - 2023/1

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AB - Lacticaseibacillus paracasei strain PS23 (PS23) exhibits some probiotic properties. In this study, a genomic analysis of PS23 revealed no genes related to virulence or antibiotic resistance. Moreover, ornithine decarboxylase activity was not detected in vitro. In addition, PS23 was sensitive to the tested antibiotics. Genotoxicity tests for PS23 including the Ames test and chromosomal aberrations in vitro using Chinese hamster ovary cells and micronuclei in immature erythrocytes of ICR mice were all negative. Moreover, following a 28-day study involving repeated oral dose toxicity tests (40, 400, and 4000 mg/kg equal 1.28 × 1010, 1.28 × 1011, and 1.28 × 1012 CFU/kg body weight, respectively) using an ICR mouse model, no adverse effects were observed from any doses. In addition, supplementation with live or heat-killed PS23 ameliorates DSS-induced colonic inflammation in mice. Our findings suggest that PS23 is safe and has anti-inflammatory effects and may therefore have therapeutic implications.

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Safety Evaluation and Anti-Inflammatory Efficacy of Lacticaseibacillus paracasei PS23

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