Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase: A chemistry-biology interdisciplinary study of the protein's structure-function- reaction mechanism relationships

Tung-Kung Wu; Cheng Hsiang Chang; Yuan Ting Liu; Tsai Ting Wang

doi:10.1002/tcr.20157

Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase: A chemistry-biology interdisciplinary study of the protein's structure-function- reaction mechanism relationships

Tung-Kung Wu^*, Cheng Hsiang Chang, Yuan Ting Liu, Tsai Ting Wang

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生物科技學系

研究成果: Review article › 同行評審

53 引文斯高帕斯（Scopus）

摘要

The oxidosqualene cyclases (EC 5.4.99-) constitute a family of enzymes that catalyze diverse cyclization/rearrangement reactions of (3S)-2,3-oxidosqualene into a distinct array of sterols and triterpenes. The relationship between the cyclization mechanism and the enzymatic structure is extremely complex and compelling. This review covers the historical achievements of biomimetic studies and current progress in structural biology, molecular genetics, and bioinformatics studies to elucidate the mechanistic and structure-function relationships of the Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase-catalyzed cyclization/rearrangement reaction.

原文	English
頁（從 - 到）	302-325
頁數	24
期刊	Chemical Record
卷	8
發行號	5
DOIs	https://doi.org/10.1002/tcr.20157
出版狀態	Published - 1 12月 2008

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10.1002/tcr.20157

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abstract = "The oxidosqualene cyclases (EC 5.4.99-) constitute a family of enzymes that catalyze diverse cyclization/rearrangement reactions of (3S)-2,3-oxidosqualene into a distinct array of sterols and triterpenes. The relationship between the cyclization mechanism and the enzymatic structure is extremely complex and compelling. This review covers the historical achievements of biomimetic studies and current progress in structural biology, molecular genetics, and bioinformatics studies to elucidate the mechanistic and structure-function relationships of the Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase-catalyzed cyclization/rearrangement reaction.",

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AB - The oxidosqualene cyclases (EC 5.4.99-) constitute a family of enzymes that catalyze diverse cyclization/rearrangement reactions of (3S)-2,3-oxidosqualene into a distinct array of sterols and triterpenes. The relationship between the cyclization mechanism and the enzymatic structure is extremely complex and compelling. This review covers the historical achievements of biomimetic studies and current progress in structural biology, molecular genetics, and bioinformatics studies to elucidate the mechanistic and structure-function relationships of the Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase-catalyzed cyclization/rearrangement reaction.

KW - Biomimetic

KW - Molecular evolution

KW - Oxidosqualene cyclase

KW - Protein engineering

KW - Protein plasticity

KW - Site-saturated mutagenesis

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Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase: A chemistry-biology interdisciplinary study of the protein's structure-function- reaction mechanism relationships

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