Risk stratification of heart failure from one drop of blood using hand-held biosensor for BNP detection

Indu Sarangadharan, Shin Li Wang, Tse Yu Tai, Anil Kumar Pulikkathodi, Chen Pin Hsu, Hui Hua Kenny Chiang, Lawrence Yu Min Liu, Yu Lin Wang*


研究成果: Article同行評審

34 引文 斯高帕斯(Scopus)


Continued risk assessment by evaluating cardiac biomarkers in healthy and unhealthy individuals can lower the mortality rate of cardiovascular diseases (CVDs). In this research, we have developed a hand-held biosensor system to rapidly screen for brain natriuretic peptide (BNP) from a single drop of whole blood. The sensor methodology is based on extended gate design of electrical double layer (EDL) field effect transistor (FET), that can directly detect BNP in whole blood, without extensive sample pre-treatments, thereby eliminating the limitations of charge screening in high ionic strength solutions. A simple sensor array chip is fabricated to integrate with the MOSFET sensor system. Sensing characteristics are elucidated using purified BNP samples in 1 × PBS (with 4% BSA), spiked BNP samples in whole blood and clinical whole blood samples. The blood cells can be gravitationally separated without the use of any external actuation. The sensor exhibits very high sensitivity over wide dynamic range of detection. The sensing characteristics are not adversely affected by the presence of background proteins or blood cells, even without gravitational blood cell separation. Thus, the biosensor system can allow users to perform rapid whole blood diagnostics with minimal user protocols, in 5 min. The features of high sensitivity, cost-effectiveness and convenience of usage empower this technology to revolutionize the mobile diagnostics and healthcare industry.

頁(從 - 到)259-265
期刊Biosensors and Bioelectronics
出版狀態Published - 1 6月 2018


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