Rhodostomin, an RGD-Containing Peptide Expressed from a Synthetic Gene in Escherichia coli, Facilitates the Attachment of Human Hepatoma Cells

Hsin Hou Chang*, Shiau Ting Hu, Tur Fu Huang, Shih Hui Chen, Yan-Hwa Wu Lee, Szecheng C.J. Lo

*此作品的通信作者

研究成果: Article同行評審

39 引文 斯高帕斯(Scopus)

摘要

Rhodostomin (Rho) from snake venom, a potent inhibitor of platelet aggregation, contains 68 amino acids having an ROD sequence and 12 cysteine residues. A chemically synthesized Rho gene was cloned and expressed in Escherichia coli. The expression of Rho gene fused with the glutathione S-transferase (GST) gene was about 10-30% of total cell proteins. The Rho-fusion protein could be recognized by antibodies raised against either a native Rho peptide or a synthetic peptide. The purified GST-Rho coated on culture plates facilitated the attachment of human hepatoma cells, which was inhibitable by co-incubation with a synthetic hexapeptide GRGDSP but not with a related peptide of GRGESP, suggesting that the E. coli-expressed Rho-fusion protein was properly folded and biologically functional.

原文English
頁(從 - 到)242-249
頁數8
期刊Biochemical and Biophysical Research Communications
190
發行號1
DOIs
出版狀態Published - 1 1月 1993

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