Revealing the cellular metabolism and microstructural changes in vivo in senescing Acer saccharum leaves using two-photon FLIM and full-field OCM

Sandeep Chakraborty, Tulsi Anna, Wen Chuan Kuo, Arthur Chiou

研究成果: Conference contribution同行評審

摘要

Seasonal as well as climate changes have immense effect on bud burst, leaf color and leaf abscission. Autumn phenology of leaves is clearly distinguishable in deciduous plant leaves where the leaf color changes from green to red (leaf senescence). In this work, two-photon fluorescence lifetime imaging microscopy (2P-FLIM) and full-field optical coherence microscopy (FF-OCM) were applied to study mitochondrial activity and microstructural changes, respectively, in the senescence of Acer saccharum (Sugar maple) leaves. Fluorescence lifetime of reduced nicotinamide adenine dinucleotide phosphate [NAD(P)H] was recorded using 2P-FLIM to quantify the cellular metabolic changes. Compared to the green leaves, the red leaves showed a 19% increase (P < 0.05) in the average fluorescence lifetime of NAD(P)H, and a 52% decrease (p < 0.005) in the free to protein-bound NAD(P)H ratio. This infers a significant change in mitochondrial metabolic regulation in red leaves in contrast to green leaves. Additionally, en-face sectional images at 0.8 μm axial resolutions of the green and the red color Acer saccharum leaves via FF-OCM using white light emitting diode (WLED) showed a well-defined microstructure of epicuticular waxy layer in green leaves as compared to red leaves where disintegrated microstructure was observed. Our approach can potentially be used to correlate mitochondrial activity with epicuticular microstructural changes in senescing leaves and other biological tissues.

原文English
主出版物標題Optics in Health Care and Biomedical Optics VII
編輯Yuguo Tang, Xingde Li, Ying Gu, Dan Zhu, Qingming Luo
發行者SPIE
ISBN(電子)9781510604674
DOIs
出版狀態Published - 2016
事件Optics in Health Care and Biomedical Optics VII - Beijing, China
持續時間: 12 10月 201614 10月 2016

出版系列

名字Proceedings of SPIE - The International Society for Optical Engineering
10024
ISSN(列印)0277-786X
ISSN(電子)1996-756X

Conference

ConferenceOptics in Health Care and Biomedical Optics VII
國家/地區China
城市Beijing
期間12/10/1614/10/16

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