Realignment process of actin stress fibers in single living cells studied by focused femtosecond laser irradiation

Ryohei Yasukuni, Jean Alexis Spitz, Rachel Meallet-Renault, Takayuki Negishi, Takuji Tada, Yoichiroh Hosokawa*, Tsuyoshi Asahi, Chisa Shukunami, Yuji Hiraki, Hiroshi Masuhara

*此作品的通信作者

研究成果: Article同行評審

8 引文 斯高帕斯(Scopus)

摘要

Three-dimensional dissection of a single actin stress fiber in a living cell was performed based on multi-photon absorption of a focused femtosecond laser pulse. The realignment process of an actin stress fiber was investigated after its direct cutting by a single-shot femtosecond laser pulse irradiation by high-speed transmission and fluorescence imaging methods. It was confirmed that mechanical force led by the femtosecond laser cutting propagates to entire cell through the cytockelton in a 100 μs time scale. The cut actin stress fiber was realigned in the time scale of a few tens of minutes. The dynamic analysis of the realignment induced by single-shot femtosecond laser gives new information on cell activity.

原文English
頁(從 - 到)6416-6419
頁數4
期刊Applied Surface Science
253
發行號15
DOIs
出版狀態Published - 30 五月 2007

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