Real-time detection of α-thrombin binding to single-strand DNA aptamers by a highly sensitive Si-based waveguide SPR biosensor

Chi Chieh Huang; Hsin Feng Hsu; Sz Hau Chen; Kun Yu Tsai; Yang Tung Huang; Chih-Sheng Lin; Shih Hsin Hsu

doi:10.1117/12.915947

Real-time detection of α-thrombin binding to single-strand DNA aptamers by a highly sensitive Si-based waveguide SPR biosensor

Chi Chieh Huang^*, Hsin Feng Hsu, Sz Hau Chen, Kun Yu Tsai, Yang Tung Huang, Chih-Sheng Lin, Shih Hsin Hsu

^*此作品的通信作者

生物科技學系

研究成果: Conference contribution › 同行評審

1 引文斯高帕斯（Scopus）

摘要

αIn this paper, real-time characterization of α-thrombin binding to single-strand DNA (ssDNA) aptamers by novel Si-based waveguide SPR biosensors has been investigated. The gold nanoparticles (AuNPs) modified with anti-thrombin antibodies were employed to bind with α-thrombin via strong antibody/antigen affinity for SPR signal amplification. The detection limit of 1 pM for -thrombin detection was achieved.

原文	English
主出版物標題	Third Asia Pacific Optical Sensors Conference
DOIs	https://doi.org/10.1117/12.915947
出版狀態	Published - 20 2月 2012
事件	3rd Asia Pacific Optical Sensors Conference - Sydney, NSW, Australia 持續時間: 31 1月 2012 → 3 2月 2012

出版系列

名字	Proceedings of SPIE - The International Society for Optical Engineering
卷	8351
ISSN（列印）	0277-786X

Conference

Conference	3rd Asia Pacific Optical Sensors Conference
國家/地區	Australia
城市	Sydney, NSW
期間	31/01/12 → 3/02/12

存取文件

10.1117/12.915947

其它文件與鏈接

Link to publication in Scopus

引用此

Huang, C. C., Hsu, H. F., Chen, S. H., Tsai, K. Y., Huang, Y. T., Lin, C-S., & Hsu, S. H. (2012). Real-time detection of α-thrombin binding to single-strand DNA aptamers by a highly sensitive Si-based waveguide SPR biosensor. 於 Third Asia Pacific Optical Sensors Conference [83512R] (Proceedings of SPIE - The International Society for Optical Engineering; 卷 8351). https://doi.org/10.1117/12.915947

@inproceedings{8a63644171e444fd97341ad3edb6b68b,

title = "Real-time detection of α-thrombin binding to single-strand DNA aptamers by a highly sensitive Si-based waveguide SPR biosensor",

abstract = "αIn this paper, real-time characterization of α-thrombin binding to single-strand DNA (ssDNA) aptamers by novel Si-based waveguide SPR biosensors has been investigated. The gold nanoparticles (AuNPs) modified with anti-thrombin antibodies were employed to bind with α-thrombin via strong antibody/antigen affinity for SPR signal amplification. The detection limit of 1 pM for -thrombin detection was achieved.",

keywords = "sensor, SPR, surface plasmon resonance",

author = "Huang, {Chi Chieh} and Hsu, {Hsin Feng} and Chen, {Sz Hau} and Tsai, {Kun Yu} and Huang, {Yang Tung} and Chih-Sheng Lin and Hsu, {Shih Hsin}",

year = "2012",

month = feb,

day = "20",

doi = "10.1117/12.915947",

language = "English",

isbn = "9780819490278",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

booktitle = "Third Asia Pacific Optical Sensors Conference",

note = "null ; Conference date: 31-01-2012 Through 03-02-2012",

}

Huang, CC, Hsu, HF, Chen, SH, Tsai, KY, Huang, YT, Lin, C-S & Hsu, SH 2012, Real-time detection of α-thrombin binding to single-strand DNA aptamers by a highly sensitive Si-based waveguide SPR biosensor. 於 Third Asia Pacific Optical Sensors Conference., 83512R, Proceedings of SPIE - The International Society for Optical Engineering, 卷 8351, 3rd Asia Pacific Optical Sensors Conference, Sydney, NSW, Australia, 31/01/12. https://doi.org/10.1117/12.915947

Real-time detection of α-thrombin binding to single-strand DNA aptamers by a highly sensitive Si-based waveguide SPR biosensor. / Huang, Chi Chieh; Hsu, Hsin Feng; Chen, Sz Hau 等.

Third Asia Pacific Optical Sensors Conference. 2012. 83512R (Proceedings of SPIE - The International Society for Optical Engineering; 卷 8351).

研究成果: Conference contribution › 同行評審

TY - GEN

T1 - Real-time detection of α-thrombin binding to single-strand DNA aptamers by a highly sensitive Si-based waveguide SPR biosensor

AU - Huang, Chi Chieh

AU - Hsu, Hsin Feng

AU - Chen, Sz Hau

AU - Tsai, Kun Yu

AU - Huang, Yang Tung

AU - Lin, Chih-Sheng

AU - Hsu, Shih Hsin

PY - 2012/2/20

Y1 - 2012/2/20

N2 - αIn this paper, real-time characterization of α-thrombin binding to single-strand DNA (ssDNA) aptamers by novel Si-based waveguide SPR biosensors has been investigated. The gold nanoparticles (AuNPs) modified with anti-thrombin antibodies were employed to bind with α-thrombin via strong antibody/antigen affinity for SPR signal amplification. The detection limit of 1 pM for -thrombin detection was achieved.

AB - αIn this paper, real-time characterization of α-thrombin binding to single-strand DNA (ssDNA) aptamers by novel Si-based waveguide SPR biosensors has been investigated. The gold nanoparticles (AuNPs) modified with anti-thrombin antibodies were employed to bind with α-thrombin via strong antibody/antigen affinity for SPR signal amplification. The detection limit of 1 pM for -thrombin detection was achieved.

KW - sensor

KW - SPR

KW - surface plasmon resonance

UR - http://www.scopus.com/inward/record.url?scp=84863180519&partnerID=8YFLogxK

U2 - 10.1117/12.915947

DO - 10.1117/12.915947

M3 - Conference contribution

AN - SCOPUS:84863180519

SN - 9780819490278

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Third Asia Pacific Optical Sensors Conference

Y2 - 31 January 2012 through 3 February 2012

ER -

Real-time detection of α-thrombin binding to single-strand DNA aptamers by a highly sensitive Si-based waveguide SPR biosensor

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Conference

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