Ratiometric temperature sensing with semiconducting polymer dots

Fangmao Ye, Changfeng Wu, Yuhui Jin, Yang-Hsiang Chan, Xuanjun Zhang, Daniel T. Chiu

研究成果: Article同行評審

339 引文 斯高帕斯(Scopus)


This communication describes ultrabright single-nanoparticle ratiometric temperature sensors based on semiconducting polymer dots (Pdots). We attached the temperature sensitive dye - Rhodamine B (RhB), whose emission intensity decreases with increasing temperature - within the matrix of Pdots. The as-prepared Pdot-RhB nanoparticle showed excellent temperature sensitivity and high brightness because it took advantage of the light harvesting and amplified energy transfer capability of Pdots. More importantly, the Pdot-RhB nanoparticle showed ratiometric temperature sensing under a single wavelength excitation and has a linear temperature sensing range that matches well with the physiologically relevant temperatures. We employed Pdot-RhB for measuring intracellular temperatures in a live-cell imaging mode. The exceptional brightness of Pdot-RhB allows this nanoscale temperature sensor to be used also as a fluorescent probe for cellular imaging.

頁(從 - 到)8146-8149
期刊Journal of the American Chemical Society
出版狀態Published - 1 6月 2011


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