@inproceedings{83eccd41cf1e49eab271f1641c09ec74,
title = "Rapid isolation and detection of methicillin-resistant Staphylococcus aureus by using a microfluidic system",
abstract = "This paper presents an integrated microfluidic system for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) by utilizing antibody-conjugated magnetic beads. By using immunomagnetic beads conjugated with specific antibodies and on-chip nucleic acid amplification, the developed microfluidic system can detect MRSA bacteria with a high sensitivity and specificity when compared with the bench-top diagnostic systems. The MRSA can be firstly bound onto the surface of the magnetic beads, followed by purifying the magnetic complexes with the incorporation of permanent magnet and the microfluidic control module. Finally, the specific genes of MRSA can be amplified by utilizing an on-chip polymerase chain reaction (PCR) module. Hence, the integrated microfluidic system can automatically perform isolation and detection of MRSA within a short period of time and can be used as a promising platform for biological and medical applications in the future.",
keywords = "MEMS, MRSA, Magnetic bead, Microfluidics, PCR",
author = "Liu, {Yu Hsin} and Lien, {Kang Yi} and Wang, {Chih Hung} and Wu, {Jiunn Jong} and Lee, {Gwo Bin}",
year = "2011",
doi = "10.1109/NEMS.2011.6017302",
language = "English",
isbn = "9781612847757",
series = "NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems",
pages = "88--91",
booktitle = "NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems",
note = "6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 ; Conference date: 20-02-2011 Through 23-02-2011",
}