Quantitative analysis of urinary stone composition with micro-Raman spectroscopy

Yi Yu Huang*, Yi Chun Chiu, Huihua Kenny Chiang, Y. H.Jet Chou, Shing Hwa Lu, Allen W. Chiu


研究成果: Conference contribution同行評審


Urolithiasis is a common, disturbing disease with high recurrent rate (60% in five years). Accurate identification of urinary stone composition is important for treatment and prevention purpose. Our previous studies have demonstrated that micro-Raman spectroscopy (MRS)-based approach successfully detects the composition of tiny stone powders after minimal invasive urological surgery. But quantitative analysis of urinary stones was not established yet. In this study, human urinary stone mixed with two compositions of COM, HAP, COD, and uric acid, were analyzed quantitatively by using a 632.98 nm Raman spectrometric system. This quantitative analysis was based on the construction of calibration curves of known mixtures of synthetically prepared pure COM, HAP, COD and uric acid. First, the various concentration (mole fraction) ratio of binary mixtures including COM and HAP, COM and COD, or COM and uric acid, were produced. Second, the intensities of the characteristic bands at 1462cm -1(IRCOM), 1477cm-1(IRCOD), 961cm-1(IRHAP) and 1402cm-1(IRuric acid), for COD, COM, HAP and uric acid were used respectively for intensity calculation. Various binary mixtures of known concentration ratio were recorded as the basis for the quantitative analysis. The ratios of the relative intensities of the Raman bands corresponding to binary mixtures of known composition on the inverse of the COM concentration yielded a linear dependence. Third, urinary stone fragments collected from patients after management were analyzed with the use of the calibration curve and the quantitative analysis of unknown samples was made by the interpolation analysis. We successfully developed a MRS-based quantitative analytical method for measuring two composition.

主出版物標題Photonic Therapeutics and Diagnostics VI
出版狀態Published - 2010
事件Photonic Therapeutics and Diagnostics VI - San Francisco, CA, United States
持續時間: 23 1月 201025 1月 2010


名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE


ConferencePhotonic Therapeutics and Diagnostics VI
國家/地區United States
城市San Francisco, CA


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