Quantifying DNA and proteins using laser-induced thermophoresis

Li Hsien Yu, Chih Hsuan Wang, Yih Fan Chen*

*此作品的通信作者

研究成果: Conference contribution同行評審

摘要

The study utilized thermophoresis, the directed motion of molecules in a temperature gradient to quantify DNA and proteins for point-of-care applications. Because the direction and speed of thermophoretic motion is dependent on the size, charge, and conformation of the molecules, the binding between molecules can induce changes in their thermophoretic motion. To quantify biomolecules using thermophoresis, we mixed fluorescently-labeled capture probes with samples and then used an infrared laser to create a temperature gradient in the solution. By adding a small fraction of polymers to the buffer solution, we accumulated the fluorescent probes in a temperature gradient using the thermophoretic effects. The thermophoretic motion of the fluorescent probes significantly changed as the target molecules bind to the specially designed capture probes. Consequently, the level of the thermophoretic accumulation, which was determined by the spatial distribution of fluorescent probes, could be used to quantify molecules. This method functioned well even when the buffer contained 10% serum, which suggested that the detection was resistant to the interferences from the molecules in serum. The thermophoresis-based detection method developed in this study only requires a laser and an epi-fluorescence microscope during the detection. Unlike many other commonly seen biosensing methods, quantifying molecules using thermophoresis does not need any fluid channels or pumps for washing away unbound molecules during the detection process. In addition, the detection does not rely on any micro- or nanofabricated chips. In short, this thermophoresis-based biosensing method can be a simple, robust, and sensitive method for quantifying proteins and DNA.

原文English
主出版物標題Frontiers in Biological Detection
主出版物子標題From Nanosensors to Systems VII
編輯Brian T. Cunningham, Benjamin L. Miller, Philippe M. Fauchet
發行者SPIE
ISBN(電子)9781628414004
DOIs
出版狀態Published - 2015
事件Frontiers in Biological Detection: From Nanosensors to Systems VII - San Francisco, United States
持續時間: 7 2月 2015 → …

出版系列

名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE
9310
ISSN(列印)1605-7422

Conference

ConferenceFrontiers in Biological Detection: From Nanosensors to Systems VII
國家/地區United States
城市San Francisco
期間7/02/15 → …

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