Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from candida tropicalis

Li Chun Chen; Sheng Cih Huang; Phimonphan Chuankhayan; Chung Der Chen; Yen Chieh Huang; Jeyaraman Jeyakanthan; Hsiao Fang Pang; Lee Chung Men; Yu Ching Chen; Yu Kuo Wang; Ming Yih Liu; Tung-Kung Wu; Chun Jung Chen

doi:10.1107/S1744309109008719

Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from candida tropicalis

Li Chun Chen, Sheng Cih Huang, Phimonphan Chuankhayan, Chung Der Chen, Yen Chieh Huang, Jeyaraman Jeyakanthan, Hsiao Fang Pang, Lee Chung Men, Yu Ching Chen, Yu Kuo Wang, Ming Yih Liu, Tung-Kung Wu^*, Chun Jung Chen

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生物科技學系

研究成果: Article › 同行評審

3 引文斯高帕斯（Scopus）

摘要

Xylose reductase (XR), which requires NADPH as a co-substrate, catalyzes the reduction of d-xylose to xylitol, which is the first step in the metabolism of d - xylose. The detailed three-dimensional structure of XR will provide a better understanding of the biological significance of XR in the efficient production of xylitol from biomass. XR of molecular mass 36.6 kDa from Candida tropicalis was crystallized using the hanging-drop vapour-diffusion method. According to X-ray diffraction data from C. tropicalis XR crystals at 2.91 Å resolution, the unit cell belongs to space group P31 or P32. Preliminary analysis indicated the presence of four XR molecules in the asymmetric unit, with 68.0% solvent content.

原文	English
頁（從 - 到）	419-421
頁數	3
期刊	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
卷	65
發行號	4
DOIs	https://doi.org/10.1107/S1744309109008719
出版狀態	Published - 15 4月 2009

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10.1107/S1744309109008719

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Chen, L. C., Huang, S. C., Chuankhayan, P., Chen, C. D., Huang, Y. C., Jeyakanthan, J., Pang, H. F., Men, L. C., Chen, Y. C., Wang, Y. K., Liu, M. Y., Wu, T-K., & Chen, C. J. (2009). Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from candida tropicalis. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 65(4), 419-421. https://doi.org/10.1107/S1744309109008719

@article{4265be9e01fc45a6a7f8f71ad54e3b1a,

title = "Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from candida tropicalis",

abstract = "Xylose reductase (XR), which requires NADPH as a co-substrate, catalyzes the reduction of d-xylose to xylitol, which is the first step in the metabolism of d - xylose. The detailed three-dimensional structure of XR will provide a better understanding of the biological significance of XR in the efficient production of xylitol from biomass. XR of molecular mass 36.6 kDa from Candida tropicalis was crystallized using the hanging-drop vapour-diffusion method. According to X-ray diffraction data from C. tropicalis XR crystals at 2.91 {\AA} resolution, the unit cell belongs to space group P31 or P32. Preliminary analysis indicated the presence of four XR molecules in the asymmetric unit, with 68.0% solvent content.",

keywords = "Candida tropicalis, Xylose reductase",

author = "Chen, {Li Chun} and Huang, {Sheng Cih} and Phimonphan Chuankhayan and Chen, {Chung Der} and Huang, {Yen Chieh} and Jeyaraman Jeyakanthan and Pang, {Hsiao Fang} and Men, {Lee Chung} and Chen, {Yu Ching} and Wang, {Yu Kuo} and Liu, {Ming Yih} and Tung-Kung Wu and Chen, {Chun Jung}",

year = "2009",

month = apr,

day = "15",

doi = "10.1107/S1744309109008719",

language = "English",

volume = "65",

pages = "419--421",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

issn = "1744-3091",

publisher = "John Wiley and Sons Ltd",

number = "4",

}

Chen, LC, Huang, SC, Chuankhayan, P, Chen, CD, Huang, YC, Jeyakanthan, J, Pang, HF, Men, LC, Chen, YC, Wang, YK, Liu, MY, Wu, T-K & Chen, CJ 2009, 'Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from candida tropicalis', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 卷 65, 編號 4, 頁 419-421. https://doi.org/10.1107/S1744309109008719

TY - JOUR

T1 - Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from candida tropicalis

AU - Chen, Li Chun

AU - Huang, Sheng Cih

AU - Chuankhayan, Phimonphan

AU - Chen, Chung Der

AU - Huang, Yen Chieh

AU - Jeyakanthan, Jeyaraman

AU - Pang, Hsiao Fang

AU - Men, Lee Chung

AU - Chen, Yu Ching

AU - Wang, Yu Kuo

AU - Liu, Ming Yih

AU - Wu, Tung-Kung

AU - Chen, Chun Jung

PY - 2009/4/15

Y1 - 2009/4/15

N2 - Xylose reductase (XR), which requires NADPH as a co-substrate, catalyzes the reduction of d-xylose to xylitol, which is the first step in the metabolism of d - xylose. The detailed three-dimensional structure of XR will provide a better understanding of the biological significance of XR in the efficient production of xylitol from biomass. XR of molecular mass 36.6 kDa from Candida tropicalis was crystallized using the hanging-drop vapour-diffusion method. According to X-ray diffraction data from C. tropicalis XR crystals at 2.91 Å resolution, the unit cell belongs to space group P31 or P32. Preliminary analysis indicated the presence of four XR molecules in the asymmetric unit, with 68.0% solvent content.

AB - Xylose reductase (XR), which requires NADPH as a co-substrate, catalyzes the reduction of d-xylose to xylitol, which is the first step in the metabolism of d - xylose. The detailed three-dimensional structure of XR will provide a better understanding of the biological significance of XR in the efficient production of xylitol from biomass. XR of molecular mass 36.6 kDa from Candida tropicalis was crystallized using the hanging-drop vapour-diffusion method. According to X-ray diffraction data from C. tropicalis XR crystals at 2.91 Å resolution, the unit cell belongs to space group P31 or P32. Preliminary analysis indicated the presence of four XR molecules in the asymmetric unit, with 68.0% solvent content.

KW - Candida tropicalis

KW - Xylose reductase

UR - http://www.scopus.com/inward/record.url?scp=64149087621&partnerID=8YFLogxK

U2 - 10.1107/S1744309109008719

DO - 10.1107/S1744309109008719

M3 - Article

C2 - 19342796

AN - SCOPUS:64149087621

SN - 1744-3091

VL - 65

SP - 419

EP - 421

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 4

ER -

Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from candida tropicalis

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