Purification, crystallization and preliminary crystallographic studies of a calmodulin-OLFp hybrid molecule

Chia Lin Chyan*, Po Chung Huang, Ta Hsien Lin, Jian Wen Huang, S. S. Lin, Hsien Bin Huang, Yi Cheng Chen

*此作品的通信作者

研究成果: Article同行評審

3 引文 斯高帕斯(Scopus)

摘要

A hybrid molecule consisting of calmodulin (CaM) and the CaM-binding domain of olfactory nucleotide-gated ion-channel peptide (CaM-OLFp) was purified and crystallized by the hanging-drop vapour-diffusion method at 298 K. The crystals diffracted to a maximum resolution of 1.85 Å at cryogenic temperature (100 K) using X-rays from a rotating anode (Cu, wavelength 1.54 Å). The crystal belongs to the monoclinic space group C2, with unit-cell parameters a = 64.76, b = 36.23, c = 70.96 Å, α = γ = 90, β = 109.4°. Analysis of the packing density shows that the asymmetric unit contains one CaM-OLFp hybrid molecule with a solvent content of 36.42%.

原文English
頁(從 - 到)785-787
頁數3
期刊Acta Crystallographica Section F: Structural Biology and Crystallization Communications
61
發行號8
DOIs
出版狀態Published - 8月 2005

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