Protocol for standardized intrathymic injection in mice

Tsung Lin Tsai; Pei Yuan Tsai; Ivan L. Dzhagalov; Chia-Lin Hsu

doi:10.1016/j.xpro.2022.102010

Protocol for standardized intrathymic injection in mice

Tsung Lin Tsai, Pei Yuan Tsai^*, Ivan L. Dzhagalov, Chia-Lin Hsu^*

^*此作品的通信作者

研究成果: Article › 同行評審

摘要

Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).¹

原文	English
文章編號	102010
期刊	STAR Protocols
卷	4
發行號	1
DOIs	https://doi.org/10.1016/j.xpro.2022.102010
出版狀態	Published - 17 3月 2023

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10.1016/j.xpro.2022.102010

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N2 - Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).1

AB - Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).1

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Protocol for standardized intrathymic injection in mice

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