Proteomic analysis of an extreme halophilic archaeon Halobacterium species NRC-1

Young Ah Goo; Eugene C. Yi; W. Andy Tao; Nitin S. Baliga; Min Pan; David R. Goodlett; Lee Hood; Wailap V. Ng

Proteomic analysis of an extreme halophilic archaeon Halobacterium species NRC-1

Young Ah Goo^*, Eugene C. Yi, W. Andy Tao, Nitin S. Baliga, Min Pan, David R. Goodlett, Lee Hood, Wailap V. Ng

^*此作品的通信作者

醫學生物技術暨檢驗學系

研究成果: Paper › 同行評審

摘要

The proteins expressed by extreme halophilic archaeon Halobacterium species NRC-1 were identified. Soluble and membrane proteins produced by the organism were collected in different fractions according to their solubility, were denatured, reduced, and finally subjected to tryptic digestion. Mass spectra were then acquired by data-dependent ion selection form a full range as well as discrete narrow survey scan m/z ranges to increase the number of identifications. It was found that a total of 442 proteins had one or more peptides with highly distinguishable MS/MS spectra and relative low noise.

原文	English
頁面	221-222
頁數	2
出版狀態	Published - 6月 2002
事件	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States 持續時間: 2 6月 2002 → 6 6月 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
國家/地區	United States
城市	Orlando, FL
期間	2/06/02 → 6/06/02

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title = "Proteomic analysis of an extreme halophilic archaeon Halobacterium species NRC-1",

abstract = "The proteins expressed by extreme halophilic archaeon Halobacterium species NRC-1 were identified. Soluble and membrane proteins produced by the organism were collected in different fractions according to their solubility, were denatured, reduced, and finally subjected to tryptic digestion. Mass spectra were then acquired by data-dependent ion selection form a full range as well as discrete narrow survey scan m/z ranges to increase the number of identifications. It was found that a total of 442 proteins had one or more peptides with highly distinguishable MS/MS spectra and relative low noise.",

author = "Goo, {Young Ah} and Yi, {Eugene C.} and Tao, {W. Andy} and Baliga, {Nitin S.} and Min Pan and Goodlett, {David R.} and Lee Hood and Ng, {Wailap V.}",

year = "2002",

month = jun,

language = "English",

pages = "221--222",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - CONF

T1 - Proteomic analysis of an extreme halophilic archaeon Halobacterium species NRC-1

AU - Goo, Young Ah

AU - Yi, Eugene C.

AU - Tao, W. Andy

AU - Baliga, Nitin S.

AU - Pan, Min

AU - Goodlett, David R.

AU - Hood, Lee

AU - Ng, Wailap V.

PY - 2002/6

Y1 - 2002/6

N2 - The proteins expressed by extreme halophilic archaeon Halobacterium species NRC-1 were identified. Soluble and membrane proteins produced by the organism were collected in different fractions according to their solubility, were denatured, reduced, and finally subjected to tryptic digestion. Mass spectra were then acquired by data-dependent ion selection form a full range as well as discrete narrow survey scan m/z ranges to increase the number of identifications. It was found that a total of 442 proteins had one or more peptides with highly distinguishable MS/MS spectra and relative low noise.

AB - The proteins expressed by extreme halophilic archaeon Halobacterium species NRC-1 were identified. Soluble and membrane proteins produced by the organism were collected in different fractions according to their solubility, were denatured, reduced, and finally subjected to tryptic digestion. Mass spectra were then acquired by data-dependent ion selection form a full range as well as discrete narrow survey scan m/z ranges to increase the number of identifications. It was found that a total of 442 proteins had one or more peptides with highly distinguishable MS/MS spectra and relative low noise.

UR - http://www.scopus.com/inward/record.url?scp=2442699182&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442699182

SP - 221

EP - 222

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Proteomic analysis of an extreme halophilic archaeon Halobacterium species NRC-1

摘要

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