Protein engineering of saccharomyces cerevisiae oxidosqualene-lanosterol cyclase into parkeol synthase

Yuan Ting Liu; Tain Chang Hu; Cheng Hsiang Chang; Wen Shiang Shie; Tung-Kung Wu

doi:10.1021/ol302341h

Protein engineering of saccharomyces cerevisiae oxidosqualene-lanosterol cyclase into parkeol synthase

Yuan Ting Liu, Tain Chang Hu, Cheng Hsiang Chang, Wen Shiang Shie, Tung-Kung Wu^*

^*此作品的通信作者

生物科技學系

研究成果: Article › 同行評審

11 引文斯高帕斯（Scopus）

摘要

A Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase mutant, ERG7^{T384Y/Q450H/V454I}, produced parkeol but not lanosterol as the sole end product. Parkeol undergoes downstream metabolism to generate compounds 9 and 10. In vitro incubation of parkeol produced a product profile similar to that of the in vivo experiment. In summary, parkeol undergoes a metabolic pathway similar to that of cycloartenol in yeast but distinct from that of lanosterol in yeast, suggesting that two different metabolic pathways of postoxidosqualene cyclization may exist in S. cerevisiae.

原文	English
頁（從 - 到）	5222-5225
頁數	4
期刊	Organic Letters
卷	14
發行號	20
DOIs	https://doi.org/10.1021/ol302341h
出版狀態	Published - 19 10月 2012

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10.1021/ol302341h

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title = "Protein engineering of saccharomyces cerevisiae oxidosqualene-lanosterol cyclase into parkeol synthase",

abstract = "A Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase mutant, ERG7T384Y/Q450H/V454I, produced parkeol but not lanosterol as the sole end product. Parkeol undergoes downstream metabolism to generate compounds 9 and 10. In vitro incubation of parkeol produced a product profile similar to that of the in vivo experiment. In summary, parkeol undergoes a metabolic pathway similar to that of cycloartenol in yeast but distinct from that of lanosterol in yeast, suggesting that two different metabolic pathways of postoxidosqualene cyclization may exist in S. cerevisiae.",

author = "Liu, {Yuan Ting} and Hu, {Tain Chang} and Chang, {Cheng Hsiang} and Shie, {Wen Shiang} and Tung-Kung Wu",

year = "2012",

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doi = "10.1021/ol302341h",

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T1 - Protein engineering of saccharomyces cerevisiae oxidosqualene-lanosterol cyclase into parkeol synthase

AU - Liu, Yuan Ting

AU - Hu, Tain Chang

AU - Chang, Cheng Hsiang

AU - Shie, Wen Shiang

AU - Wu, Tung-Kung

PY - 2012/10/19

Y1 - 2012/10/19

N2 - A Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase mutant, ERG7T384Y/Q450H/V454I, produced parkeol but not lanosterol as the sole end product. Parkeol undergoes downstream metabolism to generate compounds 9 and 10. In vitro incubation of parkeol produced a product profile similar to that of the in vivo experiment. In summary, parkeol undergoes a metabolic pathway similar to that of cycloartenol in yeast but distinct from that of lanosterol in yeast, suggesting that two different metabolic pathways of postoxidosqualene cyclization may exist in S. cerevisiae.

AB - A Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase mutant, ERG7T384Y/Q450H/V454I, produced parkeol but not lanosterol as the sole end product. Parkeol undergoes downstream metabolism to generate compounds 9 and 10. In vitro incubation of parkeol produced a product profile similar to that of the in vivo experiment. In summary, parkeol undergoes a metabolic pathway similar to that of cycloartenol in yeast but distinct from that of lanosterol in yeast, suggesting that two different metabolic pathways of postoxidosqualene cyclization may exist in S. cerevisiae.

UR - http://www.scopus.com/inward/record.url?scp=84867719751&partnerID=8YFLogxK

U2 - 10.1021/ol302341h

DO - 10.1021/ol302341h

M3 - Article

C2 - 23043506

AN - SCOPUS:84867719751

SN - 1523-7060

VL - 14

SP - 5222

EP - 5225

JO - Organic Letters

JF - Organic Letters

IS - 20

ER -

Protein engineering of saccharomyces cerevisiae oxidosqualene-lanosterol cyclase into parkeol synthase

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