Protein constituent contributes to the hypotensive and vasorelaxant activities of Cordyceps sinensis

Wen Fei Chiou*, Pei Ching Chang, Cheng Jen Chou, Chieh Fu Chen

*此作品的通信作者

研究成果: Article同行評審

96 引文 斯高帕斯(Scopus)

摘要

Cordyceps sinensis is a herb medicine in China for the treatment of general debility after sickness and for persons of advanced age. In the present study, cordyceps sinensis was extract by phosphate buffer saline (PBS) and dialyzed overnight against PBS using a membrane cut off at 3,500 dalton molecular weight. The resulting macromolecule fraction (defined as CS) was assayed in anesthetized rats for hypotensive effects and in isolated aorta for vasorelaxant effects. Intravenous injection of CS (8,16, 24 and 32 mg/kg, respectively) suppressed significantly the mean arterial pressure (MAP) in a dose-dependent manner. 32 mg/kg of CS induces the maximal hypotensive response with a 58 ± 4 mm Hg (from 107 ± 6 to 49±3 mm Hg) change in MAP and a over 45 min action duration. In aortic rings precontracted with phenylephrine treatment with CS between 0.5 and 500 μg/ml induced dose dependent relaxation. Maximal vasorelaxant response evoked by 150 μg/ml CS was 68.9±7.3 %. Furthermore, CS-induced vasorelaxation is mediated by the endothelium possibly by stimulating the release of the nitric oxide and endothelium-derived hyperpolarizing factor. In conclusion, the present study revealed that presence of a constituent in CS which reduces MAP by relaxing the vascular beds directly. However, the effect may be caused by a single active ingredient or by the combined action of many active agents found in the extract.

原文English
頁(從 - 到)1369-1376
頁數8
期刊Life Sciences
66
發行號14
DOIs
出版狀態Published - 25 2月 2000

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