Preparation and properties of insolubilized restriction endonucleases

Yan-Hwa Wu Lee; Robert W. Blakesley; Leonard A. Smith; Jack G. Chirikjian

doi:10.1093/nar/5.3.679

Preparation and properties of insolubilized restriction endonucleases

Yan-Hwa Wu Lee^*, Robert W. Blakesley, Leonard A. Smith, Jack G. Chirikjian

^*此作品的通信作者

生物科技學系

研究成果: Article › 同行評審

13 引文斯高帕斯（Scopus）

摘要

Type II restriction endonucleases Bam HI and Eco RI were covalently coupled to Sepharose. These insolubilized enzymes generated fragment patterns for several viral DNAs identical to those produced by the respective free enzymes. Conditions for optimal activity were similar for both bound and unbound forms of the enzymes. Insolubilization improved thermal stability of Bam HI and Eco RI. The bound enzyme can be recovered from reaction mixtures and reused several times. Upon storage at 4°C, coupled endonucleases remained stable for several months.

原文	English
頁（從 - 到）	679-689
頁數	11
期刊	Nucleic acids research
卷	5
發行號	3
DOIs	https://doi.org/10.1093/nar/5.3.679
出版狀態	Published - 1 3月 1978

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AU - Wu Lee, Yan-Hwa

AU - Blakesley, Robert W.

AU - Smith, Leonard A.

AU - Chirikjian, Jack G.

PY - 1978/3/1

Y1 - 1978/3/1

N2 - Type II restriction endonucleases Bam HI and Eco RI were covalently coupled to Sepharose. These insolubilized enzymes generated fragment patterns for several viral DNAs identical to those produced by the respective free enzymes. Conditions for optimal activity were similar for both bound and unbound forms of the enzymes. Insolubilization improved thermal stability of Bam HI and Eco RI. The bound enzyme can be recovered from reaction mixtures and reused several times. Upon storage at 4°C, coupled endonucleases remained stable for several months.

AB - Type II restriction endonucleases Bam HI and Eco RI were covalently coupled to Sepharose. These insolubilized enzymes generated fragment patterns for several viral DNAs identical to those produced by the respective free enzymes. Conditions for optimal activity were similar for both bound and unbound forms of the enzymes. Insolubilization improved thermal stability of Bam HI and Eco RI. The bound enzyme can be recovered from reaction mixtures and reused several times. Upon storage at 4°C, coupled endonucleases remained stable for several months.

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U2 - 10.1093/nar/5.3.679

DO - 10.1093/nar/5.3.679

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VL - 5

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JO - Nucleic acids research

JF - Nucleic acids research

IS - 3

ER -

Preparation and properties of insolubilized restriction endonucleases

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