Precise Control of Intracellular Trafficking and Receptor-Mediated Endocytosis in Living Cells and Behaving Animals

Shiau Chi Chen; Neng Jie Zeng; Grace Y. Liu; Hsien Chu Wang; Tzu Ying Lin; Yi Ling Tai; Chiao Yun Chen; Yin Fang; Yi Chien Chuang; Ching Lin Kao; Hsuan Cheng; Bing Huang Wu; Pin Chiao Sun; Odvogmed Bayansan; Yu Ting Chiu; Chi Hsuan Shih; Wen Hong Chung; Jia Bin Yang; Lily Hui Ching Wang; Po Han Chiang; Chun Hao Chen; Oliver I. Wagner; Yi Ching Wang; Yu Chun Lin

doi:10.1002/advs.202405568

Precise Control of Intracellular Trafficking and Receptor-Mediated Endocytosis in Living Cells and Behaving Animals

Shiau Chi Chen, Neng Jie Zeng, Grace Y. Liu, Hsien Chu Wang, Tzu Ying Lin, Yi Ling Tai, Chiao Yun Chen, Yin Fang, Yi Chien Chuang, Ching Lin Kao, Hsuan Cheng, Bing Huang Wu, Pin Chiao Sun, Odvogmed Bayansan, Yu Ting Chiu, Chi Hsuan Shih, Wen Hong Chung, Jia Bin Yang, Lily Hui Ching Wang, Po Han ChiangChun Hao Chen, Oliver I. Wagner, Yi Ching Wang, Yu Chun Lin^*

^*此作品的通信作者

生醫工程研究所

研究成果: Article › 同行評審

1 引文斯高帕斯（Scopus）

摘要

Intracellular trafficking, an extremely complex network, dynamically orchestrates nearly all cellular activities. A versatile method that enables the manipulation of target transport pathways with high spatiotemporal accuracy in vitro and in vivo is required to study how this network coordinates its functions. Here, a new method called RIVET (Rapid Immobilization of target Vesicles on Engaged Tracks) is presented. Utilizing inducible dimerization between target vesicles and selective cytoskeletons, RIVET can spatiotemporally halt numerous intracellular trafficking pathways within seconds in a reversible manner. Its highly specific perturbations allow for the real-time dissection of the dynamic relationships among different trafficking pathways. Moreover, RIVET is capable of inhibiting receptor-mediated endocytosis. This versatile system can be applied from the cellular level to whole organisms. RIVET opens up new avenues for studying intracellular trafficking under various physiological and pathological conditions and offers potential strategies for treating trafficking-related disorders.

原文	English
文章編號	2405568
期刊	Advanced Science
卷	11
發行號	45
DOIs	https://doi.org/10.1002/advs.202405568
出版狀態	Published - 4 12月 2024

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Chen, S. C., Zeng, N. J., Liu, G. Y., Wang, H. C., Lin, T. Y., Tai, Y. L., Chen, C. Y., Fang, Y., Chuang, Y. C., Kao, C. L., Cheng, H., Wu, B. H., Sun, P. C., Bayansan, O., Chiu, Y. T., Shih, C. H., Chung, W. H., Yang, J. B., Wang, L. H. C., ... Lin, Y. C. (2024). Precise Control of Intracellular Trafficking and Receptor-Mediated Endocytosis in Living Cells and Behaving Animals. Advanced Science, 11(45), 文章 2405568. https://doi.org/10.1002/advs.202405568

@article{5729ad99df1e478d96e8090f1630c040,

title = "Precise Control of Intracellular Trafficking and Receptor-Mediated Endocytosis in Living Cells and Behaving Animals",

abstract = "Intracellular trafficking, an extremely complex network, dynamically orchestrates nearly all cellular activities. A versatile method that enables the manipulation of target transport pathways with high spatiotemporal accuracy in vitro and in vivo is required to study how this network coordinates its functions. Here, a new method called RIVET (Rapid Immobilization of target Vesicles on Engaged Tracks) is presented. Utilizing inducible dimerization between target vesicles and selective cytoskeletons, RIVET can spatiotemporally halt numerous intracellular trafficking pathways within seconds in a reversible manner. Its highly specific perturbations allow for the real-time dissection of the dynamic relationships among different trafficking pathways. Moreover, RIVET is capable of inhibiting receptor-mediated endocytosis. This versatile system can be applied from the cellular level to whole organisms. RIVET opens up new avenues for studying intracellular trafficking under various physiological and pathological conditions and offers potential strategies for treating trafficking-related disorders.",

keywords = "chemical-inducible dimerization, cytoskeleton, optogenetics, receptor-mediated endocytosis, vesicular trafficking, virus entry",

author = "Chen, {Shiau Chi} and Zeng, {Neng Jie} and Liu, {Grace Y.} and Wang, {Hsien Chu} and Lin, {Tzu Ying} and Tai, {Yi Ling} and Chen, {Chiao Yun} and Yin Fang and Chuang, {Yi Chien} and Kao, {Ching Lin} and Hsuan Cheng and Wu, {Bing Huang} and Sun, {Pin Chiao} and Odvogmed Bayansan and Chiu, {Yu Ting} and Shih, {Chi Hsuan} and Chung, {Wen Hong} and Yang, {Jia Bin} and Wang, {Lily Hui Ching} and Chiang, {Po Han} and Chen, {Chun Hao} and Wagner, {Oliver I.} and Wang, {Yi Ching} and Lin, {Yu Chun}",

note = "Publisher Copyright: {\textcopyright} 2024 The Author(s). Advanced Science published by Wiley-VCH GmbH.",

year = "2024",

month = dec,

day = "4",

doi = "10.1002/advs.202405568",

language = "English",

volume = "11",

journal = "Advanced Science",

issn = "2198-3844",

publisher = "Wiley-VCH Verlag",

number = "45",

}

Chen, SC, Zeng, NJ, Liu, GY, Wang, HC, Lin, TY, Tai, YL, Chen, CY, Fang, Y, Chuang, YC, Kao, CL, Cheng, H, Wu, BH, Sun, PC, Bayansan, O, Chiu, YT, Shih, CH, Chung, WH, Yang, JB, Wang, LHC, Chiang, PH, Chen, CH, Wagner, OI, Wang, YC & Lin, YC 2024, 'Precise Control of Intracellular Trafficking and Receptor-Mediated Endocytosis in Living Cells and Behaving Animals', Advanced Science, 卷 11, 編號 45, 2405568. https://doi.org/10.1002/advs.202405568

TY - JOUR

T1 - Precise Control of Intracellular Trafficking and Receptor-Mediated Endocytosis in Living Cells and Behaving Animals

AU - Chen, Shiau Chi

AU - Zeng, Neng Jie

AU - Liu, Grace Y.

AU - Wang, Hsien Chu

AU - Lin, Tzu Ying

AU - Tai, Yi Ling

AU - Chen, Chiao Yun

AU - Fang, Yin

AU - Chuang, Yi Chien

AU - Kao, Ching Lin

AU - Cheng, Hsuan

AU - Wu, Bing Huang

AU - Sun, Pin Chiao

AU - Bayansan, Odvogmed

AU - Chiu, Yu Ting

AU - Shih, Chi Hsuan

AU - Chung, Wen Hong

AU - Yang, Jia Bin

AU - Wang, Lily Hui Ching

AU - Chiang, Po Han

AU - Chen, Chun Hao

AU - Wagner, Oliver I.

AU - Wang, Yi Ching

AU - Lin, Yu Chun

PY - 2024/12/4

Y1 - 2024/12/4

N2 - Intracellular trafficking, an extremely complex network, dynamically orchestrates nearly all cellular activities. A versatile method that enables the manipulation of target transport pathways with high spatiotemporal accuracy in vitro and in vivo is required to study how this network coordinates its functions. Here, a new method called RIVET (Rapid Immobilization of target Vesicles on Engaged Tracks) is presented. Utilizing inducible dimerization between target vesicles and selective cytoskeletons, RIVET can spatiotemporally halt numerous intracellular trafficking pathways within seconds in a reversible manner. Its highly specific perturbations allow for the real-time dissection of the dynamic relationships among different trafficking pathways. Moreover, RIVET is capable of inhibiting receptor-mediated endocytosis. This versatile system can be applied from the cellular level to whole organisms. RIVET opens up new avenues for studying intracellular trafficking under various physiological and pathological conditions and offers potential strategies for treating trafficking-related disorders.

AB - Intracellular trafficking, an extremely complex network, dynamically orchestrates nearly all cellular activities. A versatile method that enables the manipulation of target transport pathways with high spatiotemporal accuracy in vitro and in vivo is required to study how this network coordinates its functions. Here, a new method called RIVET (Rapid Immobilization of target Vesicles on Engaged Tracks) is presented. Utilizing inducible dimerization between target vesicles and selective cytoskeletons, RIVET can spatiotemporally halt numerous intracellular trafficking pathways within seconds in a reversible manner. Its highly specific perturbations allow for the real-time dissection of the dynamic relationships among different trafficking pathways. Moreover, RIVET is capable of inhibiting receptor-mediated endocytosis. This versatile system can be applied from the cellular level to whole organisms. RIVET opens up new avenues for studying intracellular trafficking under various physiological and pathological conditions and offers potential strategies for treating trafficking-related disorders.

KW - chemical-inducible dimerization

KW - cytoskeleton

KW - optogenetics

KW - receptor-mediated endocytosis

KW - vesicular trafficking

KW - virus entry

UR - http://www.scopus.com/inward/record.url?scp=85206140744&partnerID=8YFLogxK

U2 - 10.1002/advs.202405568

DO - 10.1002/advs.202405568

M3 - Article

C2 - 39401410

AN - SCOPUS:85206140744

SN - 2198-3844

VL - 11

JO - Advanced Science

JF - Advanced Science

IS - 45

M1 - 2405568

ER -

Precise Control of Intracellular Trafficking and Receptor-Mediated Endocytosis in Living Cells and Behaving Animals

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