Potential of faecal RNA in diagnosing colorectal cancer

Shung Haur Yang; Chih Cheng Chien; Chan Wei Chen; Shih Yun Li; Chi Jung Huang

doi:10.1016/j.canlet.2004.11.005

Potential of faecal RNA in diagnosing colorectal cancer

Shung Haur Yang, Chih Cheng Chien, Chan Wei Chen, Shih Yun Li, Chi Jung Huang^*

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醫學系外科學科

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13 引文斯高帕斯（Scopus）

摘要

Early diagnosis for colorectal cancer (CRC) by monitoring of gastrointestinal epithelial cells is a possible direct approach. Although human faeces form a heterogeneous mixture of gastrointestinal mucosal epithelial cells and other materials, we have developed a method to purify total RNA from human stool samples. The gene for faecal cytokeratin 19 (CK19) was highly expressed in stools from patients with metastatic CRC, but not from patients with non-metastatic CRC or from normal individuals. Thus, purified faecal RNA can be used for the detection of differentially expressed genes. This technique may help identifying meaningful faecal RNA markers for the non-invasive screening of patients with CRC.

原文	English
頁（從 - 到）	55-63
頁數	9
期刊	Cancer Letters
卷	226
發行號	1
DOIs	https://doi.org/10.1016/j.canlet.2004.11.005
出版狀態	Published - 8 8月 2005

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title = "Potential of faecal RNA in diagnosing colorectal cancer",

abstract = "Early diagnosis for colorectal cancer (CRC) by monitoring of gastrointestinal epithelial cells is a possible direct approach. Although human faeces form a heterogeneous mixture of gastrointestinal mucosal epithelial cells and other materials, we have developed a method to purify total RNA from human stool samples. The gene for faecal cytokeratin 19 (CK19) was highly expressed in stools from patients with metastatic CRC, but not from patients with non-metastatic CRC or from normal individuals. Thus, purified faecal RNA can be used for the detection of differentially expressed genes. This technique may help identifying meaningful faecal RNA markers for the non-invasive screening of patients with CRC.",

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AU - Yang, Shung Haur

AU - Chien, Chih Cheng

AU - Chen, Chan Wei

AU - Li, Shih Yun

AU - Huang, Chi Jung

PY - 2005/8/8

Y1 - 2005/8/8

N2 - Early diagnosis for colorectal cancer (CRC) by monitoring of gastrointestinal epithelial cells is a possible direct approach. Although human faeces form a heterogeneous mixture of gastrointestinal mucosal epithelial cells and other materials, we have developed a method to purify total RNA from human stool samples. The gene for faecal cytokeratin 19 (CK19) was highly expressed in stools from patients with metastatic CRC, but not from patients with non-metastatic CRC or from normal individuals. Thus, purified faecal RNA can be used for the detection of differentially expressed genes. This technique may help identifying meaningful faecal RNA markers for the non-invasive screening of patients with CRC.

AB - Early diagnosis for colorectal cancer (CRC) by monitoring of gastrointestinal epithelial cells is a possible direct approach. Although human faeces form a heterogeneous mixture of gastrointestinal mucosal epithelial cells and other materials, we have developed a method to purify total RNA from human stool samples. The gene for faecal cytokeratin 19 (CK19) was highly expressed in stools from patients with metastatic CRC, but not from patients with non-metastatic CRC or from normal individuals. Thus, purified faecal RNA can be used for the detection of differentially expressed genes. This technique may help identifying meaningful faecal RNA markers for the non-invasive screening of patients with CRC.

KW - Colorectal cancer

KW - Cytokeratin 19

KW - Differential expressed genes

KW - Faecal total RNA

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AN - SCOPUS:21744448994

SN - 0304-3835

VL - 226

SP - 55

EP - 63

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

Potential of faecal RNA in diagnosing colorectal cancer

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