TY - GEN
T1 - Photonic crystal enhanced fluorescence using a quartz substrate to reduce limits of detection
AU - Pokhriyal, Anusha
AU - Lu, Meng
AU - Chaudhery, Vikram
AU - Huang, Cheng Sheng
AU - Schulz, Stephen
AU - Cunningham, Brian T.
PY - 2011
Y1 - 2011
N2 - A Photonic Crystal surface fabricated upon a quartz substrate using nanoimprint lithography has been demonstrated with a signal enhancement factor as high as 7500× for the detection of LD-700 dye spin-coated upon the PC. The detection of spotted Alexa-647 labeled polypeptide on the PC exhibits a 330× SNR improvement. Using dose-response characterization of deposited fluorophore-tagged protein spots, the PCEF surface demonstrated a 140× lower limit of detection compared to a conventional glass substrate.
AB - A Photonic Crystal surface fabricated upon a quartz substrate using nanoimprint lithography has been demonstrated with a signal enhancement factor as high as 7500× for the detection of LD-700 dye spin-coated upon the PC. The detection of spotted Alexa-647 labeled polypeptide on the PC exhibits a 330× SNR improvement. Using dose-response characterization of deposited fluorophore-tagged protein spots, the PCEF surface demonstrated a 140× lower limit of detection compared to a conventional glass substrate.
UR - http://www.scopus.com/inward/record.url?scp=85088760529&partnerID=8YFLogxK
U2 - 10.1364/cleo_si.2011.cthq1
DO - 10.1364/cleo_si.2011.cthq1
M3 - Conference contribution
AN - SCOPUS:85088760529
SN - 9781557529107
T3 - Optics InfoBase Conference Papers
BT - CLEO
PB - Optical Society of America (OSA)
T2 - CLEO: Science and Innovations, CLEO_SI 2011
Y2 - 1 May 2011 through 6 May 2011
ER -