Photoluminescent graphene quantum dots for in vivo imaging of apoptotic cells

Prathik Roy, Arun Prakash Periasamy, Chiu Ya Lin, Guor Mour Her, Wei Jane Chiu, Chi Lin Li, Chia Lun Shu, Chih Ching Huang*, Chi Te Liang, Huan Tsung Chang


研究成果: Article同行評審

94 引文 斯高帕斯(Scopus)


Apoptosis (programmed cell death) is linked to many incurable neurodegenerative, cardiovascular and cancer causing diseases. Numerous methods have been developed for imaging apoptotic cells in vitro; however, there are few methods available for imaging apoptotic cells in live animals (in vivo). Here we report a novel method utilizing the unique photoluminescence properties of plant leaf-derived graphene quantum dots (GQDs) modified with annexin V antibody (AbA5) to form (AbA5)-modified GQDs (AbA5-GQDs) enabling us to label apoptotic cells in live zebrafish (Danio rerio). The key is that zebrafish shows bright red photoluminescence in the presence of apoptotic cells. The toxicity of the GQDs has also been investigated with the GQDs exhibiting high biocompatibility as they were excreted from the zebrafish's body without affecting its growth significantly at a concentration lower than 2 mg mL-1 over a period of 4 to 72 hour post fertilization. The GQDs have further been used to image human breast adenocarcinoma cell line (MCF-7 cells), human cervical cancer cell line (HeLa cells), and normal human mammary epithelial cell line (MCF-10A). These results are indispensable to further the advance of graphene-based nanomaterials for biomedical applications.

頁(從 - 到)2504-2510
出版狀態Published - 14 2月 2015


深入研究「Photoluminescent graphene quantum dots for in vivo imaging of apoptotic cells」主題。共同形成了獨特的指紋。