@article{b1e2d76b520c4113a9c9d7fcb8160b90,
title = "Photobleaching under single photon and multi-photon excitation: Chloroplasts in protoplasts from Arabidopsis thaliana",
abstract = "Photobleaching as a result of intense laser irradiation has been an important issue in the applications of confocal microscopy. In this study we are employing time-lapsed micro-spectroscopy to compare the effects of photobleaching under single photon and multi-photon excitation. A model is derived to account for photobleaching in both cases and protoplasts from Arabidopsis thaliana are employed as the sample. We have found that multi-photon excitation have impacts on the samples in ways much different from cw illumination in the linear domain.",
keywords = "Chloroplasts, Micro-spectroscopy, Multi-phpton excition, Photobleaching",
author = "Kao, {Fu Jen} and Wang, {Yi Min} and Chen, {Jian Cheng} and Cheng, {Ping Chin} and Chen, {Rung Wu} and Lin, {Bai Ling}",
note = "Funding Information: We are grateful for the generous research support from National Science Council (NSC89-2112-M-110-016 and NSC-89-2216-E-110-003) and Ministry of Education (89-B-FA08-1-4).",
year = "2002",
month = jan,
day = "1",
doi = "10.1016/S0030-4018(01)01679-0",
language = "English",
volume = "201",
pages = "85--91",
journal = "Optics Communications",
issn = "0030-4018",
publisher = "Elsevier B.V.",
number = "1-3",
}