Pharmacological study on angusticeps-type toxins from mamba snake venoms

C. Y. Lee, M. C. Tsai, M. L. Tsaur, W. W. Lin, F. H. Carlsson, F. J. Joubert

研究成果: Article同行評審

22 引文 斯高帕斯(Scopus)

摘要

Five angusticeps-type toxins, F7, F8 and C10S2C2 from Dendroaspis angusticeps and C and FS2 from D. polylepis polylepis, were tested for action on the chick biventer cervicis nerve-muscle, the frog rectus abdominis muscle and the mouse phrenic nerve-diaphragm preparations. In the chick muscle, none of these toxins exhibited any stimulatory effect up to 100 μg/ml. In the frog muscle, the response to acetylcholine, but not to carbachol, was enhanced dose dependently by F7 and C. No appreciable effect was observed with the other three toxins. In the mouse diaphragm, also only F7 and C augmented responses to indirect stimulation and produced spontaneous fasciculations. On tetanic stimulation, a marked Wedensky inhibition was observed. Their stimulatory effect was abolished by d-tubocurarine. In the presence of d-tubocurarine as well as in the denervated mouse diaphragm, neither toxin increased responses to direct stimulation. In low-calcium (0.6 mM) or high magnesium (4.2 mM) medium, the stimulatory effect of both toxins was markedly attenuated. The resting membrane potential of the mouse diaphragm was not changed. The amplitude and frequency of MEPPs and the quantal content and the half-decay time of EPPs was increased. Both toxins also produced a stimulatory effect on the isolated guinea-pig ileum, which was abolished by atropine. In the rat atrial preparation, both toxins caused negative inotropic and chronotropic effects, which were reversed by atropine. If pretreated with atropine, these effects were completely prevented. Both F7 and C markedly inhibited the cholinesterase activity of the homogenized mouse diaphragm and frog rectus abdominis muscle but not that of the chick biventer cervicis muscle. From these results, it is concluded that the angusticeps-type subgroup I toxins F7 and C stimulate both skeletal and intestinal smooth muscles and inhibit cardiac muscle by their anticholinesterase activity, as well as possibly by facilitating acetylcholine release from cholinergic nerve terminals.

原文English
頁(從 - 到)491-498
頁數8
期刊Journal of Pharmacology and Experimental Therapeutics
233
發行號2
出版狀態Published - 1985

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